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限制性内切酶作为研究4-S-乙磺酰基环磷酰胺对已知序列DNA作用的工具的应用。

Application of restriction endonucleases as a tool for studying the action of 4-S-ethanolsulfido-cyclophosphamide on DNA with known sequences.

作者信息

Lindemann H

出版信息

J Cancer Res Clin Oncol. 1985;110(2):170-2. doi: 10.1007/BF00402734.

DOI:10.1007/BF00402734
PMID:2995400
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC12252701/
Abstract

Lambda-DNA and plasmid pBR 322-DNA, respectively, were treated in vitro with increasing amounts of 4-S-ethanolsulfido-cyclophosphamide (CPA-P1). Subsequent digestion with restriction endonuclease Pvu II or Mbo II revealed discrete alterations in the cleavage patterns as compared to the controls, indicating subtle changes in DNA structure due to CPA-P1 interaction. In the case of pBR 322-DNA the CPA-P1 treatment of supercoiled circular DNA was more inhibitory to subsequent digestion as compared to the treatment of linearized plasmid DNA molecules.

摘要

分别用逐渐增加剂量的4-S-乙磺酰基环磷酰胺（CPA-P1）对λ-DNA和质粒pBR 322-DNA进行体外处理。与对照相比，随后用限制性内切酶Pvu II或Mbo II消化显示切割模式有明显改变，表明由于CPA-P1相互作用导致DNA结构发生细微变化。就pBR 322-DNA而言，与线性化质粒DNA分子的处理相比，超螺旋环状DNA的CPA-P1处理对随后的消化更具抑制作用。

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本文引用的文献

1
[Interaction of the three alkylating drugs, cyclophosphamide, ifosfamide and trofosfamide, with DNA and DNA-constituents in vitro (author's transl)].三种烷化剂药物（环磷酰胺、异环磷酰胺和曲磷胺）在体外与DNA及DNA成分的相互作用（作者译）
Arzneimittelforschung. 1980;30(12):2075-80.
2
Interaction of cyclophosphamide with DNA in isolated rat liver cell nuclei.环磷酰胺与离体大鼠肝细胞核中DNA的相互作用。
Anticancer Res. 1984 Jan-Apr;4(1-2):53-8.
3
A cautionary note on the use of certain restriction endonucleases with methylated substrates.关于某些限制性核酸内切酶与甲基化底物使用的警示说明。
Gene. 1980 Oct;11(1-2):169-71. doi: 10.1016/0378-1119(80)90097-9.
4
DNA protection with the DNA methylase M . BbvI from Bacillus brevis var. GB against cleavage by the restriction endonucleases PstI and PvuII.来自短短芽孢杆菌变种GB的DNA甲基化酶M.BbvI对DNA的保护作用，以抵抗限制性内切酶PstI和PvuII的切割。
Gene. 1980 Jul;10(2):105-12. doi: 10.1016/0378-1119(80)90128-6.

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