State Key Laboratory of Bioelectronics, Southeast University, Nanjing 210096, China.
J Biomed Nanotechnol. 2013 Feb;9(2):312-7. doi: 10.1166/jbn.2013.1493.
A rapid detection method of Pseudomonas aeruginosa based on magnetic separation and chemiluminescence was developed in this paper. Magnetic nanoparticles (MNPs) were prepared by solvothermal method with PEG-4000 as a surfactant, and then were modified. The prepared MNPs present a uniform morphology and good dispersion. The sizes of MNPs can be controlled by adjusting the dosage of FeCl3 x 6H2O. The obtained particles were characterized with Scanning electron microscope (SEM), Transmission electronic microscopy (TEM) and Fourier transform infrared (FTIR). The biotin-dUTP-labeled DNA fragments of gyrB gene were amplified by polymerase chain reaction (PCR), and Pseudomonas aeruginosa was successfully detected with detection limit as low as 7.5 fM of gyrB fragments.
本文建立了一种基于磁分离和化学发光的快速检测铜绿假单胞菌的方法。采用溶剂热法，以聚乙二醇 4000 为表面活性剂制备了磁性纳米粒子（MNPs），然后对其进行修饰。所制备的 MNPs 呈现均匀的形态和良好的分散性。通过调节 FeCl3 x 6H2O 的用量可以控制 MNPs 的尺寸。采用扫描电子显微镜（SEM）、透射电子显微镜（TEM）和傅里叶变换红外光谱（FTIR）对获得的颗粒进行了表征。通过聚合酶链反应（PCR）扩增了 gyrB 基因的生物素-dUTP 标记的 DNA 片段，成功地检测到了铜绿假单胞菌，gyrB 片段的检测限低至 7.5 fM。
