Center for Environmental Research & Children's Health (CERCH), School of Public Health, University of California, Berkeley, CA 94720, USA.
Epigenetics Research Laboratory, Department of Obstetrics & Gynecology, Duke University Medical Center, Durham, NC 27708, USA.
Epigenomics. 2018 Jul;10(7):1011-1026. doi: 10.2217/epi-2017-0178. Epub 2018 Jun 29.
Imprinted genes exhibit expression in a parent-of-origin-dependent manner and are critical for child development. Recent limited evidence suggests that prenatal exposure to phthalates, ubiquitous endocrine disruptors, can affect their epigenetic dysregulation.
MATERIALS & METHODS: We quantified DNA methylation of nine imprinted gene differentially methylated regions by pyrosequencing in 296 cord blood DNA samples in a Mexican-American cohort. Fetal exposure was estimated by phthalate metabolite concentrations in maternal urine samples during pregnancy.
Several differentially methylated regions of imprinted genes were associated with high molecular weight phthalates. The most consistent, positive, and false discovery rate significant associations were observed for MEG3.
Phthalate exposure in utero may affect methylation status of imprinted genes in newborn children.
印迹基因表现出亲本来源依赖性表达，对儿童发育至关重要。最近有限的证据表明，产前暴露于普遍存在的内分泌干扰物邻苯二甲酸酯可能会影响其表观遗传失调。
我们通过焦磷酸测序技术在一个墨西哥裔美国人队列的 296 个脐带血 DNA 样本中定量分析了 9 个印迹基因差异甲基化区域的 DNA 甲基化。通过母亲在怀孕期间尿液样本中的邻苯二甲酸代谢物浓度来估计胎儿的暴露情况。
印迹基因的几个差异甲基化区域与高分子量邻苯二甲酸酯有关。最一致、最显著且经错误发现率校正后仍有统计学意义的关联是在 MEG3 上观察到的。
子宫内邻苯二甲酸酯暴露可能会影响新生儿儿童印迹基因的甲基化状态。
