Rosen C A, Sodroski J G, Haseltine W A
Proc Natl Acad Sci U S A. 1985 Oct;82(19):6502-6. doi: 10.1073/pnas.82.19.6502.
The location of cis-acting regulatory regions within the long terminal repeat (LTR) of the human T-cell leukemia virus type I (HTLV-1) was determined. The sequences present between nucleotides -350 and -55 (cap site +1) contain an enhancer element that is active in lymphoid and nonlymphoid cell lines. The sequences located near the "TATA" and RNA initiation sites contain a promoter, the activity of which can be augmented by homologous and heterologous enhancer elements. A region responsive to trans-acting transcription factors present in HTLV-I- and HTLV type II-infected cells is located between nucleotides -159 and +315. HTLV-I LTR deletion mutants respond in a similar manner both to the trans-acting factors present in infected cells and to the tat protein encoded by the x-lor region of the genome, thus providing further evidence that the tat protein mediates transcriptional trans-activation of the LTR in HTLV-infected cells.
确定了I型人类T细胞白血病病毒（HTLV-1）长末端重复序列（LTR）内顺式作用调节区的位置。核苷酸-350至-55（帽位点+1）之间的序列包含一个增强子元件，该元件在淋巴样和非淋巴样细胞系中具有活性。位于“TATA”和RNA起始位点附近的序列包含一个启动子，同源和异源增强子元件可增强其活性。对HTLV-I和HTLV-II感染细胞中存在的反式作用转录因子有反应的区域位于核苷酸-159至+315之间。HTLV-I LTR缺失突变体对感染细胞中存在的反式作用因子和基因组x-lor区域编码的tat蛋白的反应方式相似，从而进一步证明tat蛋白介导HTLV感染细胞中LTR的转录反式激活。
