[中国湖州分离的一株重组GⅠ型诺如病毒的全基因组分析]

[Complete Genome Analysis of a Recombinant GI Norovirus Identified in Huzhou,China].

作者信息

Ji Lei, Chen Liping, Wu Xiaofang, Xu Deshun, Han Jiankang

出版信息

Bing Du Xue Bao. 2016 May;32(3):263-6.

Abstract

To study the genotype and genomic and molecular organization of a GI norovirus isolate from Huzhou, China, the genomic sequence of 2008/Huzhou/N11 was amplified by RT-PCR, then the PCR product was purified,sequenced, and input into phylogenetic and Simplot analyses. The complete genomic sequence of the 2008/Huzhou/N11 strain was 7691nucleotides (nt) long. It contained three open reading frames(ORFs):ORF1,ORF2,and ORF3,with lengths of 5367,1623,and 630 nt, respectively. Phylogenetic analysis revealed that the RdRp region of 2008/Huzhou/N11 belonged to genotype GI.2,and the VP1 and VP2regions belonged to genotype GI.6.The SimPlot analysis indicated that potential recombination points in 2008/Huzhou/N11 were located upstream of the ORF1/ORF2 overlap. The complete genomic sequence of this recombinant GI.2/GI.6 strain can serve as a reference sequence for the phylogenetic analysis of norovirus evolution.

摘要

为研究来自中国湖州的一株肠道诺如病毒分离株的基因型以及基因组和分子结构，通过逆转录聚合酶链反应（RT-PCR）扩增2008/Huzhou/N11的基因组序列，随后对PCR产物进行纯化、测序，并进行系统发育分析和Simplot分析。2008/Huzhou/N11毒株的完整基因组序列长度为7691个核苷酸（nt）。它包含三个开放阅读框（ORF）：ORF1、ORF2和ORF3，长度分别为5367、1623和630 nt。系统发育分析显示，2008/Huzhou/N11的RNA依赖的RNA聚合酶（RdRp）区域属于GI.2基因型，而病毒蛋白1（VP1）和病毒蛋白2（VP2）区域属于GI.6基因型。Simplot分析表明，2008/Huzhou/N11中的潜在重组位点位于ORF1/ORF2重叠区域的上游。这种重组的GI.2/GI.6毒株的完整基因组序列可作为诺如病毒进化系统发育分析的参考序列。