Heilongjiang Academy of Agricultural Sciences Postdoctoral Programme, Harbin, 150086, Heilongjiang, China.
Institute of Plant Protection, Heilongjiang Academy of Agricultural Sciences, No. 368 Xuefu Road, Nangang District, Harbin, 150086, Heilongjiang, China.
World J Microbiol Biotechnol. 2018 Jul 3;34(8):109. doi: 10.1007/s11274-018-2481-0.
Glutathione-S-transferase (GST) genes exist widely in plants and play major role in metabolic detoxification of exogenous chemical substances and oxidative stress. In this study, 14 sunflower GST genes (HaGSTs) were identified based on the sunflower transcriptome database that we had constructed. Full-length cDNA of 14 HaGTSs were isolated from total RNA by reverse transcription PCR (RT-PCR). Sunflower was received biotic stress (Sclerotinia sclerotiorum) and abiotic stress (NaCl, low-temperature, drought and wound). GST activity was measured by using the universal substrate. The results showed that most of the HaGSTs were up-regulated after NaCl and PEG6000-induced stresses, while a few HaGSTs were up-regulated after S. sclerotiorum, hypothermia and wound-induced stressed, and there was correlation between the changes of GST activity and the expression of HaGSTs, indicating that HaGSTs may play regulatory role in the biotic and abiotic stress responses. 14 HaGSTs from sunflower were identified, and the expression of HaGSTs were tissue-specific and played regulatory roles in both stress and abiotic stress.
谷胱甘肽-S-转移酶（GST）基因广泛存在于植物中，在代谢解毒外源化学物质和氧化应激方面发挥主要作用。本研究基于我们构建的向日葵转录组数据库，鉴定了 14 个向日葵 GST 基因（HaGSTs）。通过反转录 PCR（RT-PCR）从总 RNA 中分离出 14 个 HaGTS 的全长 cDNA。向日葵受到生物胁迫（核盘菌）和非生物胁迫（NaCl、低温、干旱和创伤）。使用通用底物测量 GST 活性。结果表明，大多数 HaGSTs 在 NaCl 和 PEG6000 诱导的胁迫后上调，而少数 HaGSTs 在核盘菌、低温和创伤诱导的胁迫后上调，GST 活性的变化与 HaGSTs 的表达之间存在相关性，表明 HaGSTs 可能在生物和非生物胁迫反应中发挥调节作用。从向日葵中鉴定出 14 个 HaGSTs，HaGSTs 的表达具有组织特异性，并在胁迫和非生物胁迫中发挥调节作用。
