Chen Zhu, Ni Wanye, Yang Caixia, Zhang Ting, Lu Shanhong, Zhao Ronghua, Mao Xiaojian, Yu Jie
College of Pharmaceutical Science, Yunnan University of Traditional Chinese Medicine, Kunming, China.
Front Pharmacol. 2018 Jun 20;9:639. doi: 10.3389/fphar.2018.00639. eCollection 2018.
Lour., a herbaceous plant in the ginger family, has been proven to be effective in treating gastrointestinal diseases. It has been listed in the Chinese Pharmacopeia as a legal source of Amomi Fructus. In our previous study, we demonstrated that treatment with extracts of prevented the development and progression of intestinal mucositis. In the current study, we aimed to verify and explain the potential beneficial effects of on inflammatory bowel disease (IBD). The effect of water extracts (WEAV) and volatile oil of (VOAV) were evaluated on the immunological role of T lymphocytes and intestinal microecology in IBD rats induced with 2,4,6-trinitrobenzenesulfonic acid (TNBS). Body weight, food intake, colon length/weight, and disease activity index (DAI) as well as tissue damage scores were evaluated. The inflammatory response to IBD was assessed by measuring the expression of myeloperoxidase, interleukin (IL)-17 (IL-17), interferon-γ (IFN-γ), IL-10, tumor necrosis factor-α (TNF-α), and transforming growth factor-β (TGF-β). The percentage of regulatory CD4 T cells in rat spleen was measured by flow cytometry and effects on the microbial community were evaluated by 16S rDNA gene sequencing. All TNBS-induced rats showed typical clinical manifestations of IBD. IBD rats in the WEAV and VOAV treatment groups were effective in relieving body weight and appetite loss. Middle and high dosage of VOAV and WEAV significantly reduced the DAI, and tissue damage scores, whereas colon weight/length ratio was increase. All rats in the WEAV and VOAV groups showed significantly decreased IFN-γ levels and increased levels of IL-10 and TGF-β. Moreover, we observed that the percentage of regulatory CD4 T cells was significantly enhanced during treatment with WEAV. In addition, administration of WEAV and VOAV effectively inhibited the release of enterogenic endotoxin, increased short-chain fatty acid-producing bacteria belonging to Firmicutes and Bacteroidetes, and decreased the abundance of Proteobacteria. Treatment with WEAV and VOAV significantly attenuated intestinal inflammation in IBD rats, which was possibly associated with its regulation on inflammatory cytokine and CD4CD25FOXP3 T cells. Moreover, WEAV and VOAV may help maintaining the balance of intestinal microecology.
豆蔻是姜科的一种草本植物，已被证明对治疗胃肠道疾病有效。它已被列入《中国药典》，作为砂仁的法定来源。在我们之前的研究中，我们证明了用其提取物治疗可预防肠道粘膜炎的发生和发展。在当前的研究中，我们旨在验证并解释其对炎症性肠病（IBD）的潜在有益作用。评估了水提取物（WEAV）和豆蔻挥发油（VOAV）对2,4,6-三硝基苯磺酸（TNBS）诱导的IBD大鼠T淋巴细胞免疫作用和肠道微生态的影响。评估了体重、食物摄入量、结肠长度/重量、疾病活动指数（DAI）以及组织损伤评分。通过测量髓过氧化物酶、白细胞介素（IL）-17（IL-17）、干扰素-γ（IFN-γ）、IL-10、肿瘤坏死因子-α（TNF-α）和转化生长因子-β（TGF-β）的表达来评估对IBD的炎症反应。通过流式细胞术测量大鼠脾脏中调节性CD4 T细胞的百分比，并通过16S rDNA基因测序评估对微生物群落的影响。所有TNBS诱导的大鼠均表现出IBD的典型临床表现。WEAV和VOAV治疗组的IBD大鼠在减轻体重和食欲丧失方面有效。中高剂量的VOAV和WEAV显著降低了DAI和组织损伤评分，而结肠重量/长度比增加。WEAV和VOAV组的所有大鼠均表现出IFN-γ水平显著降低，IL-10和TGF-β水平升高。此外，我们观察到在WEAV治疗期间调节性CD4 T细胞的百分比显著增加。此外，给予WEAV和VOAV可有效抑制肠源性内毒素的释放，增加属于厚壁菌门和拟杆菌门的短链脂肪酸产生菌，并降低变形菌门的丰度。用WEAV和VOAV治疗可显著减轻IBD大鼠的肠道炎症，这可能与其对炎性细胞因子和CD4CD25FOXP3 T细胞的调节有关。此外，WEAV和VOAV可能有助于维持肠道微生态的平衡。
