Zhu Hongkang, Wang Ruoyong, Hua Hanyi, Qian He, Du Peng
State Key Laboratory of Food Science and Technology, Jiangnan University, Wuxi, Jiangsu, China.
Collaborative Innovation Center of Food Safety and Quality Control in Jiangsu Province, Jiangnan University, Wuxi, Jiangsu, China.
Front Nutr. 2022 Oct 12;9:1004174. doi: 10.3389/fnut.2022.1004174. eCollection 2022.
A growing number of nutraceuticals and cosmeceuticals have been utilized for millennia as anti-fatigue supplements in folk medicine. However, the anti-fatigue mechanism underlying is still far from being clearly explained. The aim of the study is to explore the underlying mechanism of the Maca compound preparation (MCP), a prescription for management of exercise-induced fatigue. In this study, mice weight-loaded swimming test was used to evaluate the anti-fatigue effect of MCP. MCP significantly improved the forelimb grip strength and Rota-rod test in behavioral tests regulating energy metabolism. 16S rDNA sequencing results showed MCP can regulate the intestinal flora at the genus level by increasing several beneficial bacteria (i.e., , and etc.), and decreasing the harmful bacteria (i.e., and ), where notable high relevance was observed between the fatigue-related biomarkers and fecal microbiota. The results of microbial function analysis suggested that MCP might improve exercise-induced fatigue by enhancing energy metabolism, carbohydrate and lipid metabolism and metabolism of terpenoids and polyketides and breakdown of amino acid metabolism. In addition, and HO-induced oxidative stress model on C2C12 cells was employed to further validate the regulation of MCP on energy metabolisms. MCP pre-treatment significantly reduced intracellular ROS accumulation, and increased glycogen content, ATP generation capacity and mitochondrial membrane potential of skeletal muscle cells, as well as conferred anti-cell necrosis ability. In conclusion, MCP plays a key role in regulating fatigue occurrence in exercising and gut microbiota balance, which may be of particular importance in the case of manual workers or sub-healthy populations.
数千年来，越来越多的营养保健品和药妆品在民间医学中被用作抗疲劳补充剂。然而，其潜在的抗疲劳机制仍远未得到清晰解释。本研究旨在探索玛咖复方制剂（MCP）这一用于治疗运动性疲劳的处方的潜在机制。在本研究中，采用小鼠负重游泳试验来评估MCP的抗疲劳效果。在调节能量代谢的行为测试中，MCP显著提高了前肢握力和转棒试验成绩。16S rDNA测序结果表明，MCP可通过增加几种有益细菌（即 、 和 等）并减少有害细菌（即 和 ）来在属水平上调节肠道菌群，其中在疲劳相关生物标志物与粪便微生物群之间观察到显著的高度相关性。微生物功能分析结果表明，MCP可能通过增强能量代谢、碳水化合物和脂质代谢以及萜类和聚酮类代谢以及氨基酸代谢的分解来改善运动性疲劳。此外，采用 和HO诱导的C2C12细胞氧化应激模型来进一步验证MCP对能量代谢的调节作用。MCP预处理显著减少了细胞内活性氧的积累，并增加了骨骼肌细胞的糖原含量、ATP生成能力和线粒体膜电位，以及赋予了抗细胞坏死能力。总之，MCP在调节运动中的疲劳发生和肠道微生物群平衡方面发挥着关键作用，这在体力劳动者或亚健康人群中可能尤为重要。
