Tangal Semih, Taşçı Yasemin, Pabuçcu Emre Göksan, Çağlar Gamze Sinem, Haliloğlu Ahmet Hakan, Yararbaş Kanay
Department of Urology, Ufuk University School of Medicine, Ankara, Turkey.
Department of Obstetrics and Gynecology, Zekai Tahir Burak Training and Research Hospital, Ankara, Turkey.
Turk J Urol. 2018 May 21;45(1):12-16. doi: 10.5152/tud.2018.96393. Print 2019 Nov.
This study was designed to evaluate the prevalence of Human Papilloma Virus (HPV) in semen and document the cycle outcomes in couples with previous intra-cytoplasmic sperm injection (ICSI) failures.
One hundred and seventeen couples with at least two ICSI attempts were included in the study. HPV infection in semen and DNA fragmentation in samples were analyzed by commercially available kits. The percentage of spermatozoa with fragmented DNA (DNA fragmentation index: DFI) was determined during fluorescence microscopic examination as previously described. The cycle outcomes of couples with or without HPV infected male partners were recorded.
According to our results, the prevalence of HPV was 7.7% in asymptomatic males with at least two previous ICSI failures. The increased DFI (>30%) was observed in 82.9% of the cases. In HPV-positive cases significantly lower number of good quality embryos were obtained. The implantation and pregnancy rates were similar in infected and non-infected males (p>0.05). The early miscarriage rate was slightly higher in HPV- positive group (33% vs. 10%, p>0.05).
In cases with previous ICSI failures, the prevalence of HPV infection in semen is not higher than previously reported infertile populations. The reproductive outcome might be impaired in HPV-positive semen due to lower number of good quality embryos, which needs to be clarified by further large population-based studies.
本研究旨在评估精液中人类乳头瘤病毒（HPV）的流行情况，并记录既往卵胞浆内单精子注射（ICSI）失败的夫妇的周期结局。
本研究纳入了117对至少进行过两次ICSI尝试的夫妇。使用市售试剂盒分析精液中的HPV感染和样本中的DNA片段化情况。如前所述，在荧光显微镜检查期间测定DNA片段化精子的百分比（DNA片段化指数：DFI）。记录有或无HPV感染男性伴侣的夫妇的周期结局。
根据我们的结果，在既往至少有两次ICSI失败的无症状男性中，HPV的流行率为7.7%。在82.9%的病例中观察到DFI增加（>30%）。在HPV阳性病例中，获得的优质胚胎数量明显减少。感染和未感染男性的着床率和妊娠率相似（p>0.05）。HPV阳性组的早期流产率略高（33%对10%，p>0.05）。
在既往ICSI失败的病例中，精液中HPV感染的流行率不高于先前报道的不育人群。HPV阳性精液可能会因优质胚胎数量减少而损害生殖结局，这需要通过进一步的大规模人群研究来阐明。
