Marycz Krzysztof, Weiss Christine, Śmieszek Agnieszka, Kornicka Katarzyna
Department of Experimental, The Faculty of Biology and Animal Science, Wroclaw University of Environmental and Life Sciences, Norwida 25, 50-375 Wroclaw, Poland.
Wroclaw Research Centre EIT+, Stablowicka 147, 54-066 Wroclaw, Poland.
Stem Cells Int. 2018 Jun 6;2018:5340756. doi: 10.1155/2018/5340756. eCollection 2018.
Mesenchymal stem cells (MSCs) are frequently used in both human and veterinary medicine because their unique properties, such as modulating the immune response and differentiating into multiple lineages, make them a valuable tool in cell-based therapies. However, many studies have indicated the age-, lifestyle-, and disease-related deterioration of MSC regenerative characteristics. However, it still needs to be elucidated how the patient's health status affects the effectiveness of MSC differentiation. In the present study, we isolated mesenchymal stem cells from adipose tissue (adipose-derived mesenchymal stem cells (ASCs)) from horses diagnosed with equine metabolic syndrome (EMS), a common metabolic disorder characterized by pathological obesity and insulin resistance. We investigated the metabolic status of isolated cells during adipogenic differentiation using multiple research methods, such as flow cytometry, PCR, immunofluorescence, or transmission and confocal microscopy. The results indicated the impaired differentiation potential of ASC Excessive ROS accumulation and ER stress are most likely the major factors limiting the multipotency of these cells. However, we observed autophagic flux during differentiation as a protective mechanism that allows cells to maintain homeostasis and remove dysfunctional mitochondria.
间充质干细胞（MSCs）因其独特的特性，如调节免疫反应和分化为多种细胞系，在人类医学和兽医学中都经常被使用，使其成为基于细胞的治疗中的一种有价值的工具。然而，许多研究表明，MSCs的再生特性会随着年龄、生活方式和疾病而恶化。然而，患者的健康状况如何影响MSCs分化的有效性仍有待阐明。在本研究中，我们从被诊断患有马代谢综合征（EMS）的马的脂肪组织中分离出间充质干细胞（脂肪来源的间充质干细胞（ASCs）），EMS是一种以病理性肥胖和胰岛素抵抗为特征的常见代谢紊乱疾病。我们使用多种研究方法，如流式细胞术、PCR、免疫荧光或透射和共聚焦显微镜，研究了分离细胞在成脂分化过程中的代谢状态。结果表明ASC的分化潜能受损。过量的活性氧积累和内质网应激很可能是限制这些细胞多能性的主要因素。然而，我们在分化过程中观察到自噬流是一种保护机制，使细胞能够维持内环境稳定并清除功能失调的线粒体。
