Papadopoulou Galini V, Maedicke Anne, Grosser Katharina, van Dam Nicole M, Martínez-Medina Ainhoa
German Centre for Integrative Biodiversity Research (iDiv) Halle-Jena-Leipzig, Molecular Interaction Ecology, Deutscher Platz, Leipzig, Germany.
Friedrich Schiller University Jena, Institute of Biodiversity, Jena, Germany.
AoB Plants. 2018 May 16;10(3):ply031. doi: 10.1093/aobpla/ply031. eCollection 2018 Jun.
Phytohormones such as jasmonic acid (JA), salicylic acid (SA), ethylene (ET) and abscisic acid (ABA) play a key role in regulation of plant immune responses to different attackers. Extensive research over recent years has led to the identification of molecular markers for specific hormonal-regulated defence pathways. However, most of our current knowledge on the regulation of plant immunity derives from studies focused on above-ground organs, mainly on the model plant . Therefore, it is unclear whether the paradigms based on experiments on above-ground organs are entirely transferable to roots. Here, we used the non-model plant to study the regulation dynamics of hormonal-related marker genes in both roots and shoots. These markers were identified in shoots after elicitation of the JA-, SA-, ET- or ABA-signalling pathways, and are commonly used to study induced responses. We assessed whether the regulation of those genes by hormonal elicitation differs between roots and shoots. To discern whether the differences in marker gene expression between roots and shoots are related to differences in hormone production or to differential responsiveness, we also measured actual hormone content in the treated tissue after elicitation. Our results show that some of the widely used markers did not show specific responsiveness to single hormone applications in . We further found that hormonal elicitation led to different response patterns of the molecular markers in shoots and roots. Our results suggest that the regulation of some hormonal-related marker genes in is organ specific and differs from the -derived paradigms.
茉莉酸（JA）、水杨酸（SA）、乙烯（ET）和脱落酸（ABA）等植物激素在调节植物对不同攻击者的免疫反应中起关键作用。近年来的广泛研究已鉴定出特定激素调节防御途径的分子标记。然而，我们目前关于植物免疫调节的大部分知识来自于对地上器官的研究，主要是在模式植物上。因此，基于地上器官实验的范式是否完全适用于根尚不清楚。在这里，我们使用非模式植物来研究根和地上部中激素相关标记基因的调控动态。这些标记是在激发JA、SA、ET或ABA信号通路后在地上部中鉴定出来的，并且通常用于研究诱导反应。我们评估了激素激发对这些基因的调控在根和地上部之间是否存在差异。为了辨别根和地上部中标记基因表达的差异是与激素产生的差异还是与不同的反应性有关，我们还测量了激发后处理组织中的实际激素含量。我们的结果表明，一些广泛使用的标记在[具体植物名称]中对单一激素处理未表现出特异性反应。我们进一步发现，激素激发导致地上部和根中分子标记的不同反应模式。我们的结果表明，[具体植物名称]中一些激素相关标记基因的调控具有器官特异性，并且与源自[模式植物名称]的范式不同。
