Pietruszka Katarzyna, Piątkowska Marta, Jedziniak Piotr
Department of Pharmacology and Toxicology, National Veterinary Research Institute, 24-100 Pulawy, Poland.
J Vet Res. 2017 Dec 27;61(4):483-487. doi: 10.1515/jvetres-2017-0071. eCollection 2017 Dec.
Ochratoxin A (OTA) is a toxic metabolite mainly produced by spp. and spp. fungi. Research on the contamination of cereals, complete feeds, and tissues with this mycotoxin has indicated that it can be a toxicological problem impacting animal health and food safety in temperate climes. OTA contamination mainly besets the global pig industry, necessitating the monitoring of feeds and animal tissues. The aim of the study was to present the results of the official monitoring of OTA in animal tissues and feeds in Poland in 2014-2016 and determine the possible correlation between the presence of OTA in different types of samples.
The presence of ochratoxin A was determined using accepted procedures based on liquid chromatography with fluorescence detection after immunoaffinity column clean-up. Determination of OTA was afforded in the range of 0.3 µg/kg to 300 µg/kg in complete feeds and from 0.2 µg/kg to 150 µg/kg in the kidneys, liver, and muscles.
Over the three year span, about 23.5% of the animal tissues samples were contaminated by ochratoxin A. In the 2014 survey, 10% of the sample tissues contained 5-10 µg/kg (only one sample above 10 μg/kg), and in 2015 and 2016, 24% of samples showed levels above the limit of quantification 0.2 μg/kg, while none of the samples exceeded the established provisional action level of 5 μg/kg for animal tissues. The animal feed analysis showed that 9% was contaminated with ochratoxin A above the limit of quantification of 0.3μg/kg. In 2% of feed samples the OTA concentration was greater than 50 μg/kg.
The results confirm the appropriacy of OTA contamination monitoring and help to increase food safety.
赭曲霉毒素A（OTA）是一种主要由曲霉属和青霉属真菌产生的有毒代谢产物。关于谷物、全价饲料和组织被这种霉菌毒素污染的研究表明，在温带气候下，它可能是一个影响动物健康和食品安全的毒理学问题。OTA污染主要困扰着全球养猪业，因此有必要对饲料和动物组织进行监测。本研究的目的是展示2014 - 2016年波兰动物组织和饲料中OTA官方监测的结果，并确定不同类型样品中OTA存在情况之间的可能相关性。
采用基于免疫亲和柱净化后荧光检测的液相色谱法，按照公认程序测定赭曲霉毒素A。全价饲料中OTA的测定范围为0.3μg/kg至300μg/kg，肾脏、肝脏和肌肉中OTA的测定范围为0.2μg/kg至150μg/kg。
在这三年期间，约23.5%的动物组织样本被赭曲霉毒素A污染。在2014年的调查中,10%的样本组织含量为5 - 10μg/kg（仅一个样本高于10μg/kg），在2015年和2016年，24%的样本含量高于定量限0.2μg/kg，而没有样本超过动物组织既定的5μg/kg临时行动水平。动物饲料分析表明，9%的饲料被赭曲霉毒素A污染且高于定量限0.3μg/kg。在2%的饲料样本中，OTA浓度大于50μg/kg。
结果证实了OTA污染监测的适当性，并有助于提高食品安全。
