McCarthy Andrew A, Barrett Ray, Beteva Antonia, Caserotto Hugo, Dobias Fabien, Felisaz Franck, Giraud Thierry, Guijarro Matias, Janocha Robert, Khadrouche Akim, Lentini Mario, Leonard Gordon A, Lopez Marrero Marcos, Malbet-Monaco Stephanie, McSweeney Sean, Nurizzo Didier, Papp Gergely, Rossi Christopher, Sinoir Jeremy, Sorez Clement, Surr John, Svensson Olof, Zander Ulrich, Cipriani Florent, Theveneau Pascal, Mueller-Dieckmann Christoph
European Molecular Biology Laboratory, Grenoble Outstation, 71 avenue des Martyrs, Grenoble 38042, France.
European Synchrotron Radiation Facility, 71 avenue des Martyrs, Grenoble 38043, France.
J Synchrotron Radiat. 2018 Jul 1;25(Pt 4):1249-1260. doi: 10.1107/S1600577518007166. Epub 2018 Jun 27.
ID30B is an undulator-based high-intensity, energy-tuneable (6.0-20 keV) and variable-focus (20-200 µm in diameter) macromolecular crystallography (MX) beamline at the ESRF. It was the last of the ESRF Structural Biology Group's beamlines to be constructed and commissioned as part of the ESRF's Phase I Upgrade Program and has been in user operation since June 2015. Both a modified microdiffractometer (MD2S) incorporating an in situ plate screening capability and a new flexible sample changer (the FlexHCD) were specifically developed for ID30B. Here, the authors provide the current beamline characteristics and detail how different types of MX experiments can be performed on ID30B (http://www.esrf.eu/id30b).
ID30B是欧洲同步辐射装置(ESRF)上基于波荡器的高强度、能量可调谐(6.0 - 20 keV)且可变焦(直径20 - 200 µm)的大分子晶体学(MX)光束线。它是ESRF结构生物学组作为ESRF一期升级计划的一部分而建造并投入使用的最后一条光束线,自2015年6月起已投入用户运行。专门为ID30B开发了一种改进的包含原位平板筛选功能的微衍射仪(MD2S)和一种新型灵活样品更换器(FlexHCD)。在此,作者介绍了当前光束线的特性,并详细说明了如何在ID30B上进行不同类型的MX实验(http://www.esrf.eu/id30b)。