• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

ID30B——欧洲同步辐射装置上用于大分子晶体学实验的多功能光束线。

ID30B - a versatile beamline for macromolecular crystallography experiments at the ESRF.

作者信息

McCarthy Andrew A, Barrett Ray, Beteva Antonia, Caserotto Hugo, Dobias Fabien, Felisaz Franck, Giraud Thierry, Guijarro Matias, Janocha Robert, Khadrouche Akim, Lentini Mario, Leonard Gordon A, Lopez Marrero Marcos, Malbet-Monaco Stephanie, McSweeney Sean, Nurizzo Didier, Papp Gergely, Rossi Christopher, Sinoir Jeremy, Sorez Clement, Surr John, Svensson Olof, Zander Ulrich, Cipriani Florent, Theveneau Pascal, Mueller-Dieckmann Christoph

机构信息

European Molecular Biology Laboratory, Grenoble Outstation, 71 avenue des Martyrs, Grenoble 38042, France.

European Synchrotron Radiation Facility, 71 avenue des Martyrs, Grenoble 38043, France.

出版信息

J Synchrotron Radiat. 2018 Jul 1;25(Pt 4):1249-1260. doi: 10.1107/S1600577518007166. Epub 2018 Jun 27.

DOI:10.1107/S1600577518007166
PMID:29979188
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC6038607/
Abstract

ID30B is an undulator-based high-intensity, energy-tuneable (6.0-20 keV) and variable-focus (20-200 µm in diameter) macromolecular crystallography (MX) beamline at the ESRF. It was the last of the ESRF Structural Biology Group's beamlines to be constructed and commissioned as part of the ESRF's Phase I Upgrade Program and has been in user operation since June 2015. Both a modified microdiffractometer (MD2S) incorporating an in situ plate screening capability and a new flexible sample changer (the FlexHCD) were specifically developed for ID30B. Here, the authors provide the current beamline characteristics and detail how different types of MX experiments can be performed on ID30B (http://www.esrf.eu/id30b).

摘要

ID30B是欧洲同步辐射装置（ESRF）上基于波荡器的高强度、能量可调谐（6.0 - 20 keV）且可变焦（直径20 - 200 µm）的大分子晶体学（MX）光束线。它是ESRF结构生物学组作为ESRF一期升级计划的一部分而建造并投入使用的最后一条光束线，自2015年6月起已投入用户运行。专门为ID30B开发了一种改进的包含原位平板筛选功能的微衍射仪（MD2S）和一种新型灵活样品更换器（FlexHCD）。在此，作者介绍了当前光束线的特性，并详细说明了如何在ID30B上进行不同类型的MX实验（http://www.esrf.eu/id30b）。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25d5/6038607/e3e8d0072e14/s-25-01249-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25d5/6038607/b7e6c6fb9195/s-25-01249-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25d5/6038607/c29bfd5a146e/s-25-01249-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25d5/6038607/c789b02060f9/s-25-01249-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25d5/6038607/a50cdb45ab3e/s-25-01249-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25d5/6038607/4c9281caec69/s-25-01249-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25d5/6038607/e3e8d0072e14/s-25-01249-fig6.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25d5/6038607/b7e6c6fb9195/s-25-01249-fig1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25d5/6038607/c29bfd5a146e/s-25-01249-fig2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25d5/6038607/c789b02060f9/s-25-01249-fig3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25d5/6038607/a50cdb45ab3e/s-25-01249-fig4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25d5/6038607/4c9281caec69/s-25-01249-fig5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/25d5/6038607/e3e8d0072e14/s-25-01249-fig6.jpg

相似文献

1
ID30B - a versatile beamline for macromolecular crystallography experiments at the ESRF.ID30B——欧洲同步辐射装置上用于大分子晶体学实验的多功能光束线。
J Synchrotron Radiat. 2018 Jul 1;25(Pt 4):1249-1260. doi: 10.1107/S1600577518007166. Epub 2018 Jun 27.
2
ID30A-3 (MASSIF-3) - a beamline for macromolecular crystallography at the ESRF with a small intense beam.ID30A - 3（MASSIF - 3）——欧洲同步辐射装置上一条用于大分子晶体学研究的具有小而强光束的光束线。
J Synchrotron Radiat. 2020 May 1;27(Pt 3):844-851. doi: 10.1107/S1600577520004002. Epub 2020 Apr 29.
3
How the ESRF helps industry and how they help the ESRF.欧洲同步辐射装置如何助力产业发展以及产业如何助力欧洲同步辐射装置。
Acta Crystallogr D Biol Crystallogr. 2013 Jul;69(Pt 7):1289-96. doi: 10.1107/S0907444913001108. Epub 2013 Jun 18.
4
A decade of user operation on the macromolecular crystallography MAD beamline ID14-4 at the ESRF.ESRF ID14-4 大分子晶体学 MAD 光束线 10 年用户操作经验
J Synchrotron Radiat. 2009 Nov;16(Pt 6):803-12. doi: 10.1107/S0909049509035377. Epub 2009 Oct 7.
5
ID29: a high-intensity highly automated ESRF beamline for macromolecular crystallography experiments exploiting anomalous scattering.ID29：利用反常散射进行大分子晶体学实验的高强度高自动化 ESRF 光束线。
J Synchrotron Radiat. 2012 May;19(Pt 3):455-61. doi: 10.1107/S0909049512009715. Epub 2012 Mar 31.
6
Upgrade of BL-5C as a highly automated macromolecular crystallography beamline at Pohang Light Source II.将BL-5C升级为浦项光源II上高度自动化的大分子晶体学光束线。
J Synchrotron Radiat. 2021 Mar 1;28(Pt 2):602-608. doi: 10.1107/S1600577521000588. Epub 2021 Feb 5.
7
BL-11C Micro-MX: a high-flux microfocus macromolecular-crystallography beamline for micrometre-sized protein crystals at Pohang Light Source II.BL-11C 微 MX：浦项光源 II 上用于微米级蛋白质晶体的高通量微焦大分子晶体学光束线
J Synchrotron Radiat. 2021 Jul 1;28(Pt 4):1210-1215. doi: 10.1107/S1600577521004355. Epub 2021 Jun 1.
8
AMX - the highly automated macromolecular crystallography (17-ID-1) beamline at the NSLS-II.NSLS-II 上的高度自动化大分子晶体学（17-ID-1）光束线 AMX。
J Synchrotron Radiat. 2022 Nov 1;29(Pt 6):1480-1494. doi: 10.1107/S1600577522009377. Epub 2022 Oct 21.
9
The ID23-1 structural biology beamline at the ESRF.欧洲同步辐射装置（ESRF）的ID23-1结构生物学光束线。
J Synchrotron Radiat. 2006 May;13(Pt 3):227-38. doi: 10.1107/S0909049506004341. Epub 2006 Apr 13.
10
ID23-2: an automated and high-performance microfocus beamline for macromolecular crystallography at the ESRF.ID23-2：ESRF 用于大分子晶体学的自动化高性能微焦斑光束线。
J Synchrotron Radiat. 2022 Mar 1;29(Pt 2):581-590. doi: 10.1107/S1600577522000984. Epub 2022 Feb 22.

引用本文的文献

1
Zymoseptoria tritici Effectors Structurally Related to Killer Proteins UmV-KP4 and UmV-KP6 Inhibit Fungal Growth, and Define Extended Protein Families in Fungi.与杀伤蛋白UmV-KP4和UmV-KP6结构相关的小麦黄斑叶枯病菌效应蛋白抑制真菌生长，并在真菌中定义了扩展蛋白家族。
Mol Plant Pathol. 2025 Aug;26(8):e70141. doi: 10.1111/mpp.70141.
2
Understanding domain swapping in the c-Src SH3 domain through hinge-loop mutagenesis.通过铰链环诱变理解c-Src SH3结构域中的结构域交换
Acta Crystallogr D Struct Biol. 2025 Sep 1;81(Pt 9):492-510. doi: 10.1107/S2059798325006977. Epub 2025 Aug 27.
3
Targeting Neuroinflammation and Cognitive Decline: First-in-Class Dual Butyrylcholinesterase and p38α Mitogen-Activated Protein Kinase Inhibitors.

本文引用的文献

1
A new MR-SAD algorithm for the automatic building of protein models from low-resolution X-ray data and a poor starting model.一种用于从低分辨率X射线数据和较差初始模型自动构建蛋白质模型的新型MR-SAD算法。
IUCrJ. 2018 Jan 25;5(Pt 2):166-171. doi: 10.1107/S2052252517017961. eCollection 2018 Mar 1.
2
FlexED8: the first member of a fast and flexible sample-changer family for macromolecular crystallography.FlexED8：大分子晶体学快速灵活的自动进样器家族的第一个成员。
Acta Crystallogr D Struct Biol. 2017 Oct 1;73(Pt 10):841-851. doi: 10.1107/S2059798317013596. Epub 2017 Sep 29.
3
Towards a compact and precise sample holder for macromolecular crystallography.
靶向神经炎症与认知衰退：一流的双重丁酰胆碱酯酶和p38α丝裂原活化蛋白激酶抑制剂
J Med Chem. 2025 Aug 28;68(16):17378-17411. doi: 10.1021/acs.jmedchem.5c00933. Epub 2025 Aug 8.
4
Current and future perspectives for structural biology at the Grenoble EPN campus: a comprehensive overview.格勒诺布尔欧洲同步辐射装置园区结构生物学的现状与未来展望：全面概述
J Synchrotron Radiat. 2025 May 1;32(Pt 3):577-594. doi: 10.1107/S1600577525002012. Epub 2025 Apr 14.
5
Unraveling the molecular mechanism of polysaccharide lyases for efficient alginate degradation.解析多糖裂解酶高效降解海藻酸盐的分子机制。
Nat Commun. 2025 Mar 18;16(1):2670. doi: 10.1038/s41467-025-56754-5.
6
Integrating In Silico and In Vitro Tools for Optimized Antibody Development-Design of Therapeutic Anti-oxMIF Antibodies.整合计算机模拟和体外工具以优化抗体开发——治疗性抗氧化型巨噬细胞迁移抑制因子抗体的设计
Antibodies (Basel). 2024 Dec 20;13(4):104. doi: 10.3390/antib13040104.
7
Hydrogen Bonding and Noncovalent Electric Field Effects in the Photoconversion of a Phytochrome.在光致变色的植光色素中氢键和非共价电场效应
J Phys Chem B. 2024 Nov 28;128(47):11644-11657. doi: 10.1021/acs.jpcb.4c06419. Epub 2024 Nov 19.
8
Substrate-dependent oxidative inactivation of a W-dependent formate dehydrogenase involving selenocysteine displacement.涉及硒代半胱氨酸置换的W依赖型甲酸脱氢酶的底物依赖性氧化失活。
Chem Sci. 2024 Jul 16;15(32):13090-13101. doi: 10.1039/d4sc02394c. eCollection 2024 Aug 14.
9
In situ serial crystallography facilitates 96-well plate structural analysis at low symmetry.原位连续结晶学有助于在低对称性下进行 96 孔板结构分析。
IUCrJ. 2024 Sep 1;11(Pt 5):780-791. doi: 10.1107/S2052252524005785.
10
Targeting the conserved active site of splicing machines with specific and selective small molecule modulators.针对剪接机器保守的活性位点，用特异性和选择性的小分子调节剂进行靶向调控。
Nat Commun. 2024 Jun 19;15(1):4980. doi: 10.1038/s41467-024-48697-0.
用于大分子晶体学的紧凑且精确的样品支架。
Acta Crystallogr D Struct Biol. 2017 Oct 1;73(Pt 10):829-840. doi: 10.1107/S2059798317013742. Epub 2017 Sep 29.
4
Structural insights into adiponectin receptors suggest ceramidase activity.脂联素受体的结构见解提示神经酰胺酶活性。
Nature. 2017 Apr 6;544(7648):120-123. doi: 10.1038/nature21714. Epub 2017 Mar 22.
5
RoboDiff: combining a sample changer and goniometer for highly automated macromolecular crystallography experiments.RoboDiff：结合样品更换器和测角器实现高度自动化的大分子晶体学实验。
Acta Crystallogr D Struct Biol. 2016 Aug;72(Pt 8):966-75. doi: 10.1107/S205979831601158X. Epub 2016 Jul 27.
6
Automated harvesting and processing of protein crystals through laser photoablation.通过激光光烧蚀实现蛋白质晶体的自动收获与处理。
Acta Crystallogr D Struct Biol. 2016 Apr;72(Pt 4):454-66. doi: 10.1107/S2059798316000954. Epub 2016 Mar 24.
7
MeshAndCollect: an automated multi-crystal data-collection workflow for synchrotron macromolecular crystallography beamlines.MeshAndCollect：一种用于同步辐射大分子晶体学光束线的自动化多晶体数据收集工作流程。
Acta Crystallogr D Biol Crystallogr. 2015 Nov;71(Pt 11):2328-43. doi: 10.1107/S1399004715017927. Epub 2015 Oct 31.
8
MASSIF-1: a beamline dedicated to the fully automatic characterization and data collection from crystals of biological macromolecules.MASSIF-1：一条专门用于对生物大分子晶体进行全自动表征和数据收集的光束线。
J Synchrotron Radiat. 2015 Nov;22(6):1540-7. doi: 10.1107/S1600577515016604. Epub 2015 Oct 3.
9
In situ X-ray data collection and structure phasing of protein crystals at Structural Biology Center 19-ID.在结构生物学中心19-ID进行蛋白质晶体的原位X射线数据收集和结构定相。
J Synchrotron Radiat. 2015 Nov;22(6):1386-95. doi: 10.1107/S1600577515016598. Epub 2015 Oct 15.
10
Combining 'dry' co-crystallization and in situ diffraction to facilitate ligand screening by X-ray crystallography.结合“干”共结晶和原位衍射以促进通过X射线晶体学进行配体筛选。
Acta Crystallogr D Biol Crystallogr. 2015 Aug;71(Pt 8):1777-87. doi: 10.1107/S1399004715010342. Epub 2015 Jul 31.