Department of NanoEngineering, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA; Materials Science and Engineering Program, University of California, San Diego, 9500 Gilman Drive, La Jolla, CA 92093, USA.
College of Liberal Arts and Sciences, National University of Defense Technology, Changsha, Hunan, 410073 PR China.
Biomaterials. 2018 Oct;179:60-70. doi: 10.1016/j.biomaterials.2018.06.027. Epub 2018 Jun 22.
Silicon carbide has been shown to be biocompatible and is used as a coating material for implanted medical devices to prevent biofilms. Silicon carbide nanomaterials are also promising in cell tracking due to their stable and strong luminescence, but more comprehensive studies of this material on the nanoscale are needed. Here, we studied the toxicity of silicon carbide nanomaterials on human mesenchymal stem cells in terms of metabolism, viability, adhesion, proliferation, migration, oxidative stress, and differentiation ability. We compared two different shapes and found that silicon carbide nanowires are toxic to human mesenchymal stem cells but not to cancer cell lines at the concentration of 0.1 mg/mL. Control silicon carbide nanoparticles were biocompatible to human mesenchymal stem cells at 0.1 mg/mL. We studied the potential mechanistic effect of silicon carbide nanowires on human mesenchymal stem cells' phenotype, cytokine secretion, and gene expression. These findings suggest that the toxic effect of silicon carbide nanomaterials to human mesenchymal stem cells are dependent on morphology.
碳化硅已被证明具有生物相容性，并被用作植入式医疗器械的涂层材料，以防止生物膜的形成。碳化硅纳米材料由于其稳定且强烈的发光性，在细胞跟踪方面也具有广阔的应用前景，但需要对这种材料进行更全面的纳米尺度研究。在这里，我们研究了碳化硅纳米材料对人骨髓间充质干细胞的代谢、活力、黏附、增殖、迁移、氧化应激和分化能力的毒性。我们比较了两种不同的形状，发现碳化硅纳米线在 0.1mg/ml 的浓度下对人骨髓间充质干细胞有毒性，但对癌细胞系没有毒性。0.1mg/ml 的对照碳化硅纳米颗粒对人骨髓间充质干细胞是生物相容的。我们研究了碳化硅纳米线对人骨髓间充质干细胞表型、细胞因子分泌和基因表达的潜在机制作用。这些发现表明，碳化硅纳米材料对人骨髓间充质干细胞的毒性作用取决于形态。
