School of Chemistry, The University of New South Wales, Sydney 2052, Australia; Australian Centre for NanoMedicine and the ARC Centre of Excellence in Convergent Bio-Nano Science and Technology, The University of New South Wales, Sydney 2052, Australia.
EMBL Australia Node in Single Molecule Science, School of Medical Sciences and the ARC Centre of Excellence in Advanced Molecular Imaging, The University of New South Wales, Sydney 2052, Australia; Allen Institute for Brain Science, Seattle, WA 98106, USA.
Biosens Bioelectron. 2018 Oct 15;117:530-536. doi: 10.1016/j.bios.2018.06.066. Epub 2018 Jul 5.
The integration of plasmonic nanoparticles into biosensors has the potential to increase the sensitivity and dynamic range of detection, through the use of single nanoparticle assays. The analysis of the localized surface plasmon resonance (LSPR) of plasmonic nanoparticles has allowed the limit of detection of biosensors to move towards single molecules. However, due to complex equipment or slow analysis times, these technologies have not been implemented for point-of-care detection. Herein, we demonstrate an advancement in LSPR analysis by presenting a technique, which utilizes an inexpensive CMOS-equipped digital camera and a dark-field microscope, that can analyse the λ of over several thousand gold nanospheres in less than a second, without the use of a spectrometer. This improves the throughput of single particle spectral analysis by enabling more nanoparticles to be probed and in a much shorter time. This technique has been demonstrated through the detection of interleukin-6 through a core-satellite binding assay. We anticipate that this technique will aid in the development of high-throughput, multiplexed and point-of-care single nanoparticle biosensors.
将等离子体纳米粒子集成到生物传感器中，通过使用单纳米粒子分析，有可能提高检测的灵敏度和动态范围。等离子体纳米粒子的局域表面等离子体共振（LSPR）分析已经使得生物传感器的检测极限朝着单分子的方向发展。然而，由于复杂的设备或缓慢的分析时间，这些技术尚未应用于即时检测。在此，我们通过提出一种技术来展示 LSPR 分析的进步，该技术利用价格低廉的 CMOS 数字相机和暗场显微镜，能够在不到一秒的时间内分析数千个金纳米球的 λ，而无需使用光谱仪。这通过允许更多的纳米粒子在更短的时间内被探测，从而提高了单粒子光谱分析的通量。该技术已经通过核心-卫星结合测定法检测白细胞介素 6 得到了验证。我们预计，这项技术将有助于开发高通量、多重和即时检测的单纳米粒子生物传感器。
