Department of Bioengineering, University of California, Los Angeles, CA 90095, USA.
Department of Bioengineering, University of California, Los Angeles, CA 90095, USA.
Biosens Bioelectron. 2019 May 1;132:162-170. doi: 10.1016/j.bios.2019.02.056. Epub 2019 Mar 4.
We demonstrate a swarm biosensing platform that detects analyte based on the change in plasmonic signal from thousands of single nanoparticles sensors, leading to increased quantitative accuracy. Following dark field microscopy, we perform computational image registration and analyses to compile the hue change from thousands of single gold nanoparticles acting as individual quantitative biosensors. This platform demonstrated a limit of detection of 10 pM with a dynamic range of at least 4 orders of magnitude in buffer solution, and the successful detection of c-reactive protein (CRP) in serum compatible with 3-tier clinical cutoffs within a 10-fold difference without the need for a blocking step. By analyzing the before-and-after status of each plasmonic sensor, our sensing scheme provides informative sensing capabilities with the flexibility to select a subset of nanoparticles with optimal performance based on their initial states. Hue comparisons within and among devices also render the platform tolerant to particle and device variation. In addition, the simplicity of the readout instrumentation based on optical imaging and the implementation of microfluidics make it promising for future adaptation into point-of-care systems.
我们展示了一种群体生物传感平台，该平台基于数千个单个纳米粒子传感器的等离子体信号变化来检测分析物，从而提高定量准确性。在暗场显微镜之后，我们进行计算图像配准和分析，以汇总作为单个定量生物传感器的数千个单个金纳米粒子的色调变化。该平台在缓冲溶液中检测限为 10 pM，动态范围至少为 4 个数量级，并且可以成功检测血清中的 C 反应蛋白（CRP），在无需阻断步骤的情况下，10 倍差异内符合 3 级临床截止值。通过分析每个等离子体传感器的前后状态，我们的传感方案提供了有信息的传感能力，并且具有根据其初始状态选择具有最佳性能的纳米粒子子集的灵活性。器件内和器件间的色调比较也使该平台能够耐受粒子和器件的变化。此外，基于光学成像的读出仪器的简单性以及微流控技术的实现使其有望适应未来的即时护理系统。
