Rosiq Saed, Hammam Olfat, Abdelalim Ahmed, Anas Amgad, Khalil Heba, Amer Mosbah
Tropical Medicine Department, Al Azhar University, Cairo, Egypt.
Pathology Department, Theodor Bilharz Research Institute, Imbaba, Giza, Egypt.
Open Access Maced J Med Sci. 2018 Jun 7;6(6):968-974. doi: 10.3889/oamjms.2018.208. eCollection 2018 Jun 20.
Colorectal cancer is the fourth common tumour in Egypt after lymphoid, breast and urinary tumours. The study aims to assess the expression of Lgr5 and CD133 in pre-malignant (adenomatous polyps and IBD), malignant colorectal lesions and normal colonic mucosa by immunohistochemical staining.
This prospective study was done on 100 patients presenting with colonic symptoms, patients were divided into four groups; group I including 20 patients in the control group, group II including 20 ulcerative colitis (U.C) patients, group III including 20 patients with adenomatous polyps and group IV including 40 patients with colorectal cancer (CRC).
Lgr5 and CD133 expression was significantly higher in carcinoma than in adenomas, IBD and normal mucosa (P < 0.001). Lrg5 and CD133 was positively correlated with histological grade (P = 0.001), depth of invasion (P = 0.001), lymph node metastasis (P < 0.001), distant metastasis (P < 0.004) and TNM stage (P < 0.001).
Role of Lgr5 and CD133 as stem cell marker was expressed and presented with different expression in the normal colonic mucosa, adenoma and CRC and showed increased expression in an advanced stage of CRC. This may suggest its possible involvement in colorectal tumorigenesis and invasion.
结直肠癌是埃及继淋巴瘤、乳腺癌和泌尿系统肿瘤之后的第四大常见肿瘤。本研究旨在通过免疫组化染色评估Lgr5和CD133在癌前病变（腺瘤性息肉和炎症性肠病）、恶性结直肠病变及正常结肠黏膜中的表达。
本前瞻性研究针对100例有结肠症状的患者展开，患者被分为四组；第一组为对照组，包含20例患者；第二组为溃疡性结肠炎（U.C）患者，共20例；第三组为腺瘤性息肉患者，有20例；第四组为结直肠癌（CRC）患者，共40例。
Lgr5和CD133在癌组织中的表达显著高于腺瘤、炎症性肠病及正常黏膜（P < 0.001）。Lrg5和CD133与组织学分级（P = 0.001）、浸润深度（P = 0.001）、淋巴结转移（P < 0.001）、远处转移（P < 0.004）及TNM分期（P < 0.001）呈正相关。
Lgr5和CD133作为干细胞标志物的作用得以体现，且在正常结肠黏膜、腺瘤及结直肠癌中呈现出不同的表达，在结直肠癌晚期表达增加。这可能提示其可能参与了结直肠癌的发生和侵袭过程。
