青海地方猪品种与商业猪品种线粒体 DNA D-环区的遗传变异与亲缘关系。
Genetic variation and relationships in the mitochondrial DNA D-loop region of Qinghai indigenous and commercial pig breeds.
机构信息
1State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining, 810016 Qinghai China.
2College of Agriculture and Animal Husbandry, Qinghai University, Xining, Qinghai China.
出版信息
Cell Mol Biol Lett. 2018 Jul 3;23:31. doi: 10.1186/s11658-018-0097-x. eCollection 2018.
BACKGROUND
Bamei pigs are special germplasm resources in Qinghai. In the specific environment of the Qinghai plateau, local breeds, through long-term natural and artificial selection, have acquired a relatively stable population structure and genetic diversity. This study revealed Bamei pigs' genetic diversity at the molecular level and the degree of introgression of commercial breeds into Bamei pigs.
METHODS
The mitochondrial DNA D-loop region was amplified and sequenced using the ABI 3130 DNA sequencer. Sequences were collected, edited and aligned using the MEGA 5.0, DNASTAR and ClustalX 1.81 software. Haplotypes of all sequences and genetic diversity were analyzed by DnaSP 5.0 software.
RESULTS AND CONCLUSIONS
We identified a total of 73 polymorphic sites in our 489 novel sequences, including 1 single variable site and 72 parsimony informative sites.Genetic diversity was analyzed in this study. The results showed that haplotype diversity, nucleotide diversity and the average number of nucleotide differences of Bamei pigs were lower than those of commercial pigs. Synthetic evaluation of genetic diversity through principal component analysis indicated that Bamei pigs also showed low genetic diversity. In summary, the results of sequence analysis showed that Bamei pigs were low in genetic diversity, and the introgression of commercial pigs into Bamei pigs was serious.
背景
八眉猪是青海特有的种质资源。在青海高原的特殊环境下，地方品种通过长期的自然和人工选择，获得了相对稳定的群体结构和遗传多样性。本研究揭示了八眉猪在分子水平上的遗传多样性和商业品种向八眉猪的渗入程度。
方法
使用 ABI 3130 DNA 测序仪扩增和测序线粒体 DNA D-环区。使用 MEGA 5.0、DNASTAR 和 ClustalX 1.81 软件收集、编辑和对齐序列。使用 DnaSP 5.0 软件分析所有序列的单倍型和遗传多样性。
结果与结论
共鉴定了 489 个新序列中的 73 个多态性位点，包括 1 个单变异位点和 72 个简约信息位点。对遗传多样性进行了分析。结果表明，八眉猪的单倍型多样性、核苷酸多样性和平均核苷酸差异数均低于商业猪。通过主成分分析对遗传多样性进行综合评价表明，八眉猪的遗传多样性也较低。综上所述，序列分析结果表明，八眉猪遗传多样性较低，商业猪向八眉猪的渗入较为严重。
Zotero 插件