Department of Pharmacology, Institute of Translational Medicine, School of Medicine , Yangzhou University , Yangzhou 225001 , Jiangsu , China.
Jiangsu Key Laboratory of Integrated Traditional Chinese and Western Medicine for Prevention and Treatment of Senile Diseases , Yangzhou 225001 , Jiangsu , China.
ACS Appl Mater Interfaces. 2018 Aug 1;10(30):25026-25036. doi: 10.1021/acsami.8b04932. Epub 2018 Jul 19.
Infections caused by bacteria are a growing global challenge for public health as bacteria develop resistance, which will cause the failure of anti-infective treatment eventually. An effective alternative strategy to traditional antibacterial therapy is utilizing reactive oxygen species (ROS) to kill bacteria. Here, we report a simple route to prepare PEGylated nitrogen-doped carbon capsules (PEG-N-CCs) as an antibacterial agent. The PEG-N-CCs can translate near-infrared light (NIR) into heat and produce a high concentration of ROS triggered by NIR irradiation. Both heating and ROS are critical to destroy the outer membranes and rupture cell bodies, causing DNA fragmentation and glutathione oxidation both in Gram-negative Escherichia coli, Gram-positive Staphylococcus aureus, and their multidrug-resistant strains. Moreover, PEG-N-CCs plus NIR irradiation can efficiently scavenge the existing biofilms and prevent the formation of new biofilms, killing planktonic bacteria as well as those within the biofilm. Our studies prove that the PEG-N-CCs plus NIR irradiation can provide a simple and effective platform for combating bacteria, employing carbon nanomaterials as an antibacterial alternative for treatment of infectious diseases.
细菌感染是全球公共卫生面临的一个日益严峻的挑战，因为细菌会产生耐药性，最终导致抗感染治疗失败。利用活性氧物质 (ROS) 杀灭细菌是一种替代传统抗菌治疗的有效策略。在这里，我们报告了一种简单的方法来制备聚乙二醇化氮掺杂碳胶囊（PEG-N-CCs）作为一种抗菌剂。PEG-N-CCs 可以将近红外光（NIR）转化为热量，并在近红外辐射下产生高浓度的 ROS。加热和 ROS 都对破坏外膜和破裂细胞体至关重要，导致革兰氏阴性大肠杆菌、革兰氏阳性金黄色葡萄球菌及其多药耐药株的 DNA 断裂和谷胱甘肽氧化。此外，PEG-N-CCs 加近红外辐射可以有效地清除现有的生物膜并防止新的生物膜形成，杀死浮游菌和生物膜内的细菌。我们的研究证明，PEG-N-CCs 加近红外辐射可以为抗菌提供一个简单有效的平台，利用碳纳米材料作为治疗传染病的抗菌替代物。
