Li Jian, Wang Bin, Luo Yingying, Bian Yajie, Wang Ruipei
1Departments of Otorhinolaryngology and Geriatrics, Cangzhou Central Hospital, 16 Xinhua West Road, Cangzhou, 061000 Hebei People's Republic of China.
Departments of Pediatric Bone Oncology, Cangzhou Combine Traditional Chinese and Western Medicine Hospital, 31 Huanghe West Road, Cangzhou, 061000 Hebei People's Republic of China.
Allergy Asthma Clin Immunol. 2018 Jun 11;14:22. doi: 10.1186/s13223-018-0249-6. eCollection 2018.
Allergic rhinitis (AR), characterized by sneezing, nasal itching and rhinorrhea, affects a large number of population. This study aimed to explore the effects of artemisinin alone or combined with neurectomy of pterygoid canal in ovalbumin-induced AR mouse model and illustrate the underlying mechanisms.
Allergic symptoms were evaluated to verify inhibitory effect of artemisinin alone or combined with neurectomy of pterygoid canal on AR. Serum levels of histamine, immunoglobulin E (IgE) and inflammatory factors TNF, INF-γ, IL-1β IL-10, IL-4 and IL-5 were measured by ELISA. The mRNA levels of TNF, INF-γ, IL-1β and IL-10 in local lymph nodes were measured by RT-qPCR. The total and phosphorylated levels of ERK and JNK were assessed by Western blot. CD4CD25Foxp3 T (Treg) cells were analyzed by flow cytometry.
Artemisinin significantly relieved the behavior symptoms of AR mice. The administration of artemisinin strikingly suppressed the expression of histamine, IgE and inflammatory factors. An increased Treg cell proportion and inhibited ERK phosphorylation were observed in artemisinin-treated groups as compared to those in the AR group. Moreover, artemisinin plus neurectomy of pterygoid almost abolished the behavioral score increase in AR mice.
These results indicated that artemisinin exhibited anti-allergic effect by inhibiting ERK activation and increasing Treg cell proportion, which subsequently decreased the expressions of allergic mediators. In addition, artemisinin combined with neurectomy of pterygoid showed better efficacy than artemisinin alone.
过敏性鼻炎（AR）以打喷嚏、鼻痒和流涕为特征，影响大量人群。本研究旨在探讨青蒿素单独或联合翼管神经切除术对卵清蛋白诱导的AR小鼠模型的影响，并阐明其潜在机制。
评估过敏症状，以验证青蒿素单独或联合翼管神经切除术对AR的抑制作用。采用酶联免疫吸附测定法（ELISA）检测血清中组胺、免疫球蛋白E（IgE）和炎性因子TNF、INF-γ、IL-1β、IL-10、IL-4和IL-5的水平。采用逆转录定量聚合酶链反应（RT-qPCR）检测局部淋巴结中TNF、INF-γ、IL-1β和IL-10的mRNA水平。采用蛋白质免疫印迹法评估ERK和JNK的总水平和磷酸化水平。采用流式细胞术分析CD4CD25Foxp3 T（Treg）细胞。
青蒿素显著缓解了AR小鼠的行为症状。青蒿素给药显著抑制了组胺、IgE和炎性因子的表达。与AR组相比，青蒿素治疗组观察到Treg细胞比例增加且ERK磷酸化受到抑制。此外，青蒿素联合翼管神经切除术几乎消除了AR小鼠行为评分的增加。
这些结果表明，青蒿素通过抑制ERK激活和增加Treg细胞比例发挥抗过敏作用，进而降低过敏介质的表达。此外，青蒿素联合翼管神经切除术比单独使用青蒿素显示出更好的疗效。
