M Nagalingam, V N Kalpana, V Devi Rajeswari, A Panneerselvam
Department of Zoology, Thiruvalluvar University, Serkadu, Vellore - 14, Tamil Nadu, India.
Department of Biomedical sciences, School of Biosciences and Technology, VIT, Vellore - 14, Tamil Nadu, India.
Biotechnol Rep (Amst). 2018 Jun 22;19:e00268. doi: 10.1016/j.btre.2018.e00268. eCollection 2018 Sep.
The objective of the study was to synthesize gold nanoparticles (Au NPs) using leaf extract of ). The biosynthesized Au NPs were characterized using UV-vis spectroscopy, X-ray Diffraction (XRD), Fourier transform infrared spectroscopy (FTIR), Scanning electron microscopy (SEM), Energy dispersive X-ray analysis (EDX), Zeta potential and Transmission electron microscopy (TEM). Morphologically, the Au NPs showed spherical shaped structures. Size distribution of Au NPs calculated using Scherrer's formula, showed an average size of 80-120 nm. Au NPs were studied for anti-bacterial and cytotoxic activities. Au NPs exhibited significant anti-microbial activity against , , , , , and by agar well diffusion method. The cytotoxic effect of the biogenic synthesized Au NPs against A549 human lung cancer cell lines provided a vigorous evidence of anticancer activity of Au NPs. Further, the toxicity study of the green synthesized Au NPs on (Zebra fish) embryo was evaluated. This study reports that colloidal Au NPs can be synthesized by simple, non-hazardous methods and that bio-synthesized Au NPs have significant therapeutic properties.
该研究的目的是使用（此处原文缺失植物名称）的叶提取物合成金纳米颗粒（Au NPs）。采用紫外可见光谱、X射线衍射（XRD）、傅里叶变换红外光谱（FTIR）、扫描电子显微镜（SEM）、能量色散X射线分析（EDX）、zeta电位和透射电子显微镜（TEM）对生物合成的Au NPs进行表征。从形态上看，Au NPs呈现出球形结构。使用谢乐公式计算的Au NPs尺寸分布显示平均尺寸为80 - 120纳米。对Au NPs的抗菌和细胞毒性活性进行了研究。通过琼脂孔扩散法，Au NPs对（此处原文缺失细菌名称）表现出显著的抗菌活性。生物合成的Au NPs对A549人肺癌细胞系的细胞毒性作用有力地证明了Au NPs的抗癌活性。此外，还评估了绿色合成的Au NPs对斑马鱼胚胎的毒性研究。该研究报告称，胶体Au NPs可以通过简单、无害方法合成，且生物合成的Au NPs具有显著的治疗特性。
