粪便微生物移植在感染后模型中有效缓解内脏敏感性。
Fecal Microbiota Transplantation Is Effective in Relieving Visceral Hypersensitivity in a Postinfectious Model.
机构信息
Division of Gastroenterology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei 430022, China.
出版信息
Biomed Res Int. 2018 Jan 30;2018:3860743. doi: 10.1155/2018/3860743. eCollection 2018.
AIM
To investigate the effect of fecal microbiota transplantation on visceral hypersensitivity compared with .
METHODS
Mice visceral hypersensitivity was induced by . After 8 weeks, they were divided into three groups (controls, , and fecal microbiota transplantation) and were daily treated by gavage with 0.2 ml PBS, HB55020, or fecal microbiota for 7 days. Visceral hypersensitivity was tested with abdominal withdrawal reflex. Permeability of colon epithelium was assessed with Ussing chamber.
RESULTS
After administration of , compared with mice in postinfectious group, mice had higher pain threshold ( < 0.05). After administration of fecal microbiota, compared with mice in postinfectious group, mice had higher pain threshold ( < 0.05). Fecal microbiota transplantation was as effective as in relieving visceral hypersensitivity. Administration of or fecal microbiota transplantation improved colon epithelium permeability. Expression of occluding-1 was increased.
CONCLUSION
Manipulation of microbiota is effective in relieving visceral hypersensitivity. Fecal microbiota transplantation is as effective as administration.
目的
与 相比，研究粪便微生物群移植对内脏敏感性的影响。
方法
通过 诱导小鼠内脏敏感性。8 周后，将其分为三组（对照组、 和粪便微生物群移植组），并每天通过灌胃给予 0.2ml PBS、HB55020 或粪便微生物群 7 天。通过腹壁退缩反射测试内脏敏感性。使用 Ussing 室评估结肠上皮通透性。
结果
给予 后，与感染后组相比，疼痛阈值较高（<0.05）。给予粪便微生物群后，与感染后组相比，疼痛阈值较高（<0.05）。粪便微生物群移植与 一样有效缓解内脏敏感性。给予 或粪便微生物群移植可改善结肠上皮通透性。occluding-1 的表达增加。
结论
微生物群的操纵在缓解内脏敏感性方面是有效的。粪便微生物群移植与 给药一样有效。
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