College of Materials Science and Engineering , Harbin University of Science and Technology , Harbin 150040 , China.
The Key Laboratory of Biomedical Information Engineering of Ministry of Education, Biomedical Engineering and Biomechanics Center , Xi'an Jiaotong University , Xi'an 710049 , China.
Biomacromolecules. 2018 Aug 13;19(8):3351-3360. doi: 10.1021/acs.biomac.8b00636. Epub 2018 Jul 23.
As one of important biomaterials for localized drug delivery system, chitosan hydrogel still suffer several challenges, including poor mechanical properties, passive drug release behavior and lack of remote stimuli response. To address these challenges, a facile in situ hybridization method was reported for fabricate tough magnetic chitosan hydrogel (MCH), which remotely switched drug release from passive release to pulsatile release under a low frequency alternating magnetic field (LAMF). The in situ hybridization method avoided the aggregation of magnetic nanoparticles (MNPs) in hydrogel, which simultaneously brings 416% and 265% increase in strength and elastic modulus, respectively. The mechanical property enhancement was contributed by the physical crosslinking of in situ synthesized MNPs. When a LAMF with 15 min ON-15 min OFF cycles was applied to MCH, the fraction release showed zigzag shape and pulsatile release behavior with quick response. The cumulative release and fraction release of drug from MCH were improved by 67.2% and 31.9%, respectively. MTT results and cell morphology indicated that the MCH have excellent biocompatibility and no acute adverse effect on MG-63 cells. The developed tough MCH system holds great potential for applications in smart drug release system with noninvasive characteristics and magnetic field stimulated drug release behavior.
作为局部药物输送系统的重要生物材料之一，壳聚糖水凝胶仍然面临着一些挑战，包括机械性能差、药物释放行为被动和缺乏远程刺激响应。为了解决这些挑战，报道了一种简便的原位杂交方法来制备坚韧的磁性壳聚糖水凝胶（MCH），该水凝胶在低频交变磁场（LAMF）下可从被动释放远程切换为脉冲释放。原位杂交方法避免了磁纳米颗粒（MNPs）在水凝胶中的聚集，这同时使强度和弹性模量分别提高了 416%和 265%。机械性能的增强是由原位合成的 MNPs 的物理交联贡献的。当施加具有 15 min ON-15 min OFF 循环的 LAMF 时，释放分数呈现锯齿形和脉冲释放行为，具有快速响应。MCH 中药物的累积释放和释放分数分别提高了 67.2%和 31.9%。MTT 结果和细胞形态表明，MCH 具有优异的生物相容性，对 MG-63 细胞没有急性不良影响。开发的坚韧 MCH 系统具有非侵入性和磁场刺激药物释放行为的特点，在智能药物释放系统中有很大的应用潜力。
