一种“猪-人-禽”重组 H1N2 猪流感病毒株的分离及其遗传变异性分析。

Isolation of a Reassortant H1N2 Swine Flu Strain of Type "Swine-Human-Avian" and Its Genetic Variability Analysis.

机构信息

Institute of Animal Husbandry and Veterinary Medicine, Fujian Academy of Agriculture Sciences, Fujian Animal Disease Control Technology Development Center, Fuzhou, Fujian 350013, China.

出版信息

Biomed Res Int. 2018 May 29;2018:1096079. doi: 10.1155/2018/1096079. eCollection 2018.

DOI:10.1155/2018/1096079

PMID:30003086

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5996443/

Abstract

We isolated an influenza strain named A/Swine/Fujian/F1/2010 (H1N2) from a pig suspected to be infected with swine flu. The results of electron microscopy, hemagglutination (HA) assay, hemagglutination inhibition (HI) assay, and whole genome sequencing analysis suggest that it was a reassortant virus of swine (H1N1 subtype), human (H3N2 subtype), and avian influenza viruses. To further study the genetic evolution of A/Swine/Fujian/F1/2010 (H1N2), we cloned its whole genome fragments using RT-PCR and performed phylogenetic analysis on the eight genes. As a result, the nucleotide sequences of HA, NA, PB1, PA, PB2, NP, M, and NS gene are similar to those of A/Swine/Shanghai/1/2007(H1N2) with identity of 98.9%, 98.9%, 99.0%, 98.6%, 99.0%, 98.9%, 99.3%, and 99.3%, respectively. Similar to A/Swine/Shanghai/1/2007(H1N2), we inferred that the HA, NP, M, and NS gene fragments of A/Swine/Fujian/F1/2010 (H1N2) strain were derived from classical swine influenza H3N2 subtype, NA and PB1 were derived from human swine influenza H3N2 subtype, and PB2 and PA genes were derived from avian influenza virus. This further validates the role of swine as a "mixer" for influenza viruses.

摘要

我们从一头疑似感染猪流感的猪身上分离出一株名为 A/Swine/Fujian/F1/2010（H1N2）的流感病毒株。电子显微镜观察、血凝（HA）试验、血凝抑制（HI）试验和全基因组测序分析结果表明，它是一种猪（H1N1 亚型）、人（H3N2 亚型）和禽流感病毒的重组病毒。为了进一步研究 A/Swine/Fujian/F1/2010（H1N2）的遗传进化，我们使用 RT-PCR 克隆了其全基因组片段，并对这 8 个基因进行了系统进化分析。结果显示，HA、NA、PB1、PA、PB2、NP、M 和 NS 基因的核苷酸序列与 A/Swine/Shanghai/1/2007（H1N2）高度相似，同源性分别为 98.9%、98.9%、99.0%、98.6%、99.0%、98.9%、99.3%和 99.3%。与 A/Swine/Shanghai/1/2007（H1N2）相似，我们推断 A/Swine/Fujian/F1/2010（H1N2）株的 HA、NP、M 和 NS 基因片段来源于经典猪源 H3N2 亚型，NA 和 PB1 来源于人源 H3N2 亚型，而 PB2 和 PA 基因来源于禽流感病毒。这进一步证实了猪作为流感病毒“混合器”的作用。