Ren Jianli, Liu Jian, Sui Xin
Department of Gastroenterology, Dezhou People's Hospital, Dezhou, China.
J BUON. 2018 May-Jun;23(3):665-671.
To study the expressions of cyclooxygenase-2 (COX-2) and matrix metalloproteinase-13 (MMP-13) genes in gastric cancer, and to investigate the correlation between them and gastric cancer and their effects onprognosis.
80 cases of tumor tissues and 40 cases of normal tumor-adjacent tissues were collected from patients with gastric cancer admitted to the Surgical Department of our hospital. The mRNA expression levels of COX-2 and MMP- 13 in tumor tissues and normal tumor-adjacent tissues were detected via real-time fluorescence reverse transcription polymerase chain reaction (RT-PCR). The expressions of COX-2 and MMP-13 in gastric cancer tissues and normal tumor-adjacent tissues were detected via immunohistochemical method. The clinical data of patients were recorded and the correlation between the COX-2 and MMP-13 expressions and the pathological parameters and prognosis of patients with gastric cancer were analyzed.
RT-PCR results showed that the mRNA expressions of COX-2 and MMP-13 in gastric cancer tissues were significantly higher than those in normal tumor-adjacent tissues. Immunohistochemical results showed that the positive expression rates of COX-2 and MMP-13 in gastric cancer tissues were 76.25% (60/80) and 71.25% (57/80), respectively and the high expression was related to the invasion, metastasis and tumor stage. The 5-year overall survival of patients was 16.6% (13/80). Single-factor survival analysis showed that both COX-2 and MMP-13 were factors influencing the overall survival of patients with gastric cancer (p<0.01).
The high expressions of COX-2 and MMP-13 are closely related to the pathological parameters of patients with gastric cancer, especially the invasion, metastasis and tumor stage. COX-2 and MMP-13 can be used as reference indexes to guide the treatment of gastric cancer and predict the disease prognosis.
研究环氧化酶-2（COX-2）和基质金属蛋白酶-13（MMP-13）基因在胃癌中的表达情况，探讨它们与胃癌的相关性及其对预后的影响。
收集我院外科收治的胃癌患者80例肿瘤组织及40例正常癌旁组织。采用实时荧光逆转录聚合酶链反应（RT-PCR）检测肿瘤组织及正常癌旁组织中COX-2和MMP-13的mRNA表达水平。采用免疫组织化学方法检测胃癌组织及正常癌旁组织中COX-2和MMP-13的表达。记录患者的临床资料，分析COX-2和MMP-13表达与胃癌患者病理参数及预后的相关性。
RT-PCR结果显示，胃癌组织中COX-2和MMP-13的mRNA表达明显高于正常癌旁组织。免疫组织化学结果显示，胃癌组织中COX-2和MMP-13的阳性表达率分别为76.25%（60/80）和71.25%（57/80），高表达与侵袭、转移及肿瘤分期有关。患者的5年总生存率为16.6%（13/80）。单因素生存分析显示，COX-2和MMP-13均为影响胃癌患者总生存的因素（p<0.01）。
COX-2和MMP-13的高表达与胃癌患者的病理参数密切相关，尤其是侵袭、转移及肿瘤分期。COX-2和MMP-13可作为指导胃癌治疗及预测疾病预后的参考指标。
