Program of Biomedical Engineering, South Dakota School of Mines and Technology, Rapid City, SD 57701, USA.
Department of Biomedical Engineering, University of South Dakota, BioSNTR, Sioux Falls, SD 57107, USA.
Colloids Surf B Biointerfaces. 2018 Nov 1;171:31-39. doi: 10.1016/j.colsurfb.2018.07.004. Epub 2018 Jul 6.
Three-dimensional (3D) scaffolds as artificial ECMs have been extensively studied to mimic the critical features of natural ECMs. To develop more clinically relevant 3D scaffolds, electrospun nanofibrous scaffolds with different weight ratios of PCL/PLA (i.e., 100/0, 60/40, and 20/80) were fabricated via the thermally induced (nanofiber) self-agglomeration (TISA) method. The hypothesis was that, with the weight ratio increase of stiffer and more bioactive PLA in the 3D PCL/PLA blend scaffolds, the osteogenic differentiation of human mesenchymal stem cells (hMSCs) would be enhanced. The results indicated that, all of the 3D scaffolds were elastic/resilient and possessed interconnected and hierarchical pores with sizes from sub-microns to ∼300 μm; therefore, the morphological structures of these scaffolds were similar to those of natural ECMs. The PLA80 scaffolds exhibited the best overall properties in terms of density, porosity, water absorption capacity, mechanical properties, bioactivity, and cell viability. Furthermore, with increasing the PLA weight ratio, the alkaline phosphatase (ALP) activity, calcium content, and gene expression level were also increased, probably due to the improved stiffness/bioactivity of scaffold. Hence, the novel 3D electrospun PLA80 nanofibrous scaffold might be desired/favorable for the osteogenic differentiation of hMSCs.
三维（3D）支架作为人工细胞外基质已被广泛研究，以模拟天然细胞外基质的关键特征。为了开发更具临床相关性的 3D 支架，通过热诱导（纳米纤维）自聚集（TISA）方法制备了具有不同聚己内酯/聚乳酸（即 100/0、60/40 和 20/80）重量比的静电纺丝纳米纤维支架。假设随着 3D PCL/PLA 共混支架中刚性和更具生物活性的 PLA 的重量比增加，人骨髓间充质干细胞（hMSCs）的成骨分化将增强。结果表明，所有 3D 支架均具有弹性/弹性，并且具有相互连接的和分级的孔，其尺寸从亚微米到约 300μm；因此，这些支架的形态结构类似于天然细胞外基质。PLA80 支架在密度、孔隙率、吸水率、力学性能、生物活性和细胞活力方面表现出最佳的整体性能。此外，随着 PLA 重量比的增加，碱性磷酸酶（ALP）活性、钙含量和基因表达水平也增加，这可能是由于支架的刚性/生物活性得到了改善。因此，新型 3D 静电纺 PLA80 纳米纤维支架可能有利于 hMSCs 的成骨分化。
