Postgraduate Program in Environmental Engineering (PPGEA), Federal University of Technology (UTFPR-AP), 86812-460 Apucarana, PR, Brazil.
School of Biomedical Engineering, Department of Mechanical Engineering, and Department of Chemical and Biological Engineering, Colorado State University, 1370 Campus Delivery, Fort Collins, CO, United States.
Carbohydr Polym. 2018 Oct 1;197:47-56. doi: 10.1016/j.carbpol.2018.05.062. Epub 2018 May 25.
Processing stable polysaccharide membranes with suitable mechanical properties has been challenging for applications in wound healing and tissue engineering. Here we expand the characterization of pectin/chitosan (PT/CS) membranes (without covalent crosslinking), which we recently reported. Membranes containing pectin (PT) excess were formed, and PT/CS ratio can be tuned to enhance the mechanical strength, and to modulate hydrophilicity and cytocompatibility. The surface wettability and swelling properties of the polyelectrolyte complexes (PECs) played an important role to promote the attachment of stem cells. These PECs membranes have ultimate tensile strength similar to that of human skin, which is on the order of ten times higher than similar previously reported polysaccharide materials. We show for the first time that these new PT/CS membranes may promote anchorage, adhesion and support human stem cell growth, making them candidate materials for tissue engineering purposes.
对于应用于伤口愈合和组织工程的领域，具有合适机械性能的稳定多糖膜的加工一直具有挑战性。在此，我们扩展了最近报道的果胶/壳聚糖（PT/CS）膜（无共价交联）的特性研究。形成了含有果胶（PT）过量的膜，并且可以调整 PT/CS 比例以增强机械强度，并调节亲水性和细胞相容性。聚电解质复合物（PEC）的表面润湿性和溶胀性能对于促进干细胞的附着起着重要作用。这些 PEC 膜的极限拉伸强度类似于人体皮肤，比以前报道的类似多糖材料高十倍左右。我们首次表明，这些新型的 PT/CS 膜可能促进人干细胞的附着、黏附和支持，使它们成为组织工程应用的候选材料。
