1 Department of Nuclear Medicine and Molecular Imaging, Suwon, Korea.
2 Department of Neurology, Ajou University School of Medicine, Suwon, Korea.
Cell Transplant. 2018 Aug;27(8):1203-1209. doi: 10.1177/0963689718788067. Epub 2018 Jul 16.
The purpose of this study was to investigate how intravenously injected bone marrow-derived mesenchymal stem cells (BMSCs) are distributed in the body of an Alzheimer's disease (AD) animal model.
Stem cells were collected from bone marrow of mice and labeled with Indium-111 (In). The In-labeled BMSCs were infused intravenously into 3×Tg-AD mice in the AD group and non-transgenic mice (B6129SF2/J) as controls. Biodistribution was evaluated with a gamma counter and gamma camera 24 and 48 h after injecting the stem cells.
A gamma count of the brain showed a higher distribution of labeled cells in the AD model than in the control group at 24 (p = .0004) and 48 h (p = .0016) after injection of the BMSCs. Similar results were observed by gamma camera imaging (i.e., brain uptake in the AD model was significantly higher than that in the control group). Among the other organs, uptake by the spleen was the highest in both groups. More BMSCs were found in the lungs of the control group than in those of the AD group.
These results suggest that more intravenously infused BMSCs reached the brain in the AD model than in the control group, but the numbers of stem cells reaching the brain was very small.
本研究旨在探讨静脉注射骨髓间充质干细胞（BMSCs）在阿尔茨海默病（AD）动物模型体内的分布情况。
从小鼠骨髓中采集干细胞，并标记铟-111（In）。将 In 标记的 BMSCs 静脉注入 AD 组的 3×Tg-AD 小鼠和非转基因小鼠（B6129SF2/J）作为对照。在注入干细胞后 24 和 48 小时，使用伽马计数器和伽马相机评估生物分布。
大脑的伽马计数显示，在注入 BMSCs 后 24 小时（p =.0004）和 48 小时（p =.0016），AD 模型中的标记细胞分布明显高于对照组。伽马相机成像也得到了类似的结果（即，AD 模型中的脑摄取明显高于对照组）。在其他器官中，脾脏的摄取量在两组中均最高。对照组的肺部比 AD 组吸收了更多的 BMSCs。
这些结果表明，与对照组相比，更多静脉注射的 BMSCs 到达了 AD 模型的大脑，但到达大脑的干细胞数量非常少。
