Pharmacognosy and Phytochemistry Department, Faculty of Pharmacy, Airlangga University, Jl. Dharmawangsa Dalam, 60286 Surabaya, Indonesia.
Pharmacognosy and Phytochemistry Department, Faculty of Pharmacy, Airlangga University, Jl. Dharmawangsa Dalam, 60286 Surabaya, Indonesia.
J Ethnopharmacol. 2018 Oct 28;225:64-70. doi: 10.1016/j.jep.2018.06.038. Epub 2018 Jul 3.
Temu giring, the rhizome of Curcuma heyneana Valeton & Zipj (C. heyneana), is native to tropical regions, especially in Indonesia. It is traditionally used as a skin care, for cosmetic and body freshness, in Javanese and Balinese women, and has many other bioactivities such as antioxidant, anticancer and antiinflammatory.
The purpose of this study was to determine the antiaging activity of C. heyneana to prove its traditional use.
The antioxidant activity was determined using the DPPH free radical method, and anti-aging activity was examined using in vitro assays such as tyrosinase inhibitor and collagenase inhibitor. In vivo tests were performed by observing histomorphologic changes in rat skin exposed to Ultraviolet (UV) rays. The total curcuminoid contents and chromatographic profiles were determined by Thin Layer Chromatography (TLC) - densitometry.
In all in vitro assays, all of the extracts showed a dose-dependent manner in the final concentration range from 62.5 to 500 µg/mL for DPPH assay and 31.25 to 250 µg/mL for tyrosinase inhibition and collagenase inhibition assay. Curcuminoid (CUR), the active principle of Curcuma genus shows antioxidant, tyrosinase inhibitor and collagenase inhibitor activity greater than all C. heyneana extracts. The in vivo assay results showed that the topical application of the crude extract of C. heyneana produced significant improvement effects on the UV-induced skin structure damage. The total CUR content was correlated with the anti-aging activity of Curcuma heyneana.
The results show that C. heyneana contains antioxidant compounds and has potent anti-aging activity, indicating that it can be used as an anti-aging drug candidate or as a phyto-cosmeceutical.
莪术，是姜黄属植物莪术（Curcuma heyneana Valeton & Zipj）的根茎，原产于热带地区，特别是印度尼西亚。在爪哇和巴厘岛妇女中，它传统上被用作皮肤护理、美容和身体清新剂，具有许多其他生物活性，如抗氧化、抗癌和抗炎作用。
本研究旨在确定莪术的抗衰老活性，以证明其传统用途。
使用 DPPH 自由基法测定抗氧化活性，使用体外试验如酪氨酸酶抑制剂和胶原酶抑制剂测定抗衰老活性。通过观察暴露于紫外线 (UV) 射线的大鼠皮肤的组织形态学变化进行体内试验。通过薄层色谱 (TLC) - 密度法测定总姜黄素含量和色谱图谱。
在所有体外试验中，所有提取物在最终浓度范围为 62.5 至 500 µg/mL（DPPH 测定）和 31.25 至 250 µg/mL（酪氨酸酶抑制和胶原酶抑制测定）之间均表现出剂量依赖性。姜黄素（CUR），姜黄属的活性成分，表现出比所有莪术提取物更强的抗氧化、酪氨酸酶抑制剂和胶原酶抑制剂活性。体内试验结果表明，莪术粗提取物的局部应用对 UV 诱导的皮肤结构损伤产生了显著的改善作用。总 CUR 含量与莪术的抗衰老活性相关。
结果表明，莪术含有抗氧化化合物，具有很强的抗衰老活性，表明它可以用作抗衰老药物候选物或植物化妆品。
