Dai Hui, Deng Hou-Bo, Wang Ya-Hong, Guo Jia-Juan
Department of Tumor and Blood Disease, Changchun University of Traditional Chinese Medicine Affiliated Hospital, Changchun, Jilin 130021, P.R. China.
Department of Liver, Spleen and Stomach Disease, Changchun University of Traditional Chinese Medicine Affiliated Hospital, Changchun, Jilin 130021, P.R. China.
Oncol Lett. 2018 Aug;16(2):1579-1583. doi: 10.3892/ol.2018.8772. Epub 2018 May 22.
The inhibitory effect of resveratrol on the growth of gastric cancer cells through downregulation of the Wnt/β-catenin signaling pathway were studied. First we determined the effective concentration of resveratrol on the growth and proliferation of MGC-803 gastric cancer cells. Methylthiazolyl tetrazolium assay showed that resveratrol significantly inhibited the proliferation of MGC-803 cells in a dose-dependent manner. Resveratrol induced apoptotic morphological changes in MGC-803 cells. Reverse transcription-polymerase chain reaction and western blot analysis showed that resveratrol downregulated the expression of three important components of the Wnt signaling pathway, β-catenin, c-myc, and cyclin D1, at the mRNA and protein levels. Overall, resveratrol inhibits the growth of MGC-803 cells by inhibiting the Wnt signaling pathway. This study provides a new idea and direction for the antitumor mechanism of resveratrol.
研究了白藜芦醇通过下调Wnt/β-连环蛋白信号通路对胃癌细胞生长的抑制作用。首先，我们确定了白藜芦醇对MGC-803胃癌细胞生长和增殖的有效浓度。甲基噻唑基四氮唑法显示，白藜芦醇以剂量依赖性方式显著抑制MGC-803细胞的增殖。白藜芦醇诱导MGC-803细胞出现凋亡形态学变化。逆转录-聚合酶链反应和蛋白质印迹分析表明，白藜芦醇在mRNA和蛋白质水平下调了Wnt信号通路的三个重要成分β-连环蛋白、c-myc和细胞周期蛋白D1的表达。总体而言，白藜芦醇通过抑制Wnt信号通路抑制MGC-803细胞的生长。本研究为白藜芦醇的抗肿瘤机制提供了新的思路和方向。
