Kimta Neetika, Chauhan Ankush, Puri Sunil, Kumari Amita, Sharma Rohit, Kumar Akshay, Kapoor Dhriti
School of Biological and Environmental Sciences, Shoolini University of Biotechnology and Management Sciences, Solan, 173229, India.
Faculty of Allied Health Sciences, Chettinad Hospital and Research Institute, Chettinad Academy of Research and Education, Kelambakkam, 603103, Tamil Nadu, India.
Appl Biochem Biotechnol. 2025 Jan;197(1):398-426. doi: 10.1007/s12010-024-05031-0. Epub 2024 Aug 13.
The green synthesis of copper oxide nanoparticles (CuO) mediated by crude ethanolic extract and the n-butanol fraction of Adiantum venustum represents a groundbreaking approach in nanotechnology, combining ecological sustainability with advanced functionality. This innovative method leverages the natural bioactive compounds present in A. venustum to produce CuO nanoparticles, which exhibit remarkable antioxidant, anti-inflammatory, antimicrobial, and anti-proliferative properties. The green synthesized nanoparticles were characterized using a variety of techniques, as XRD confirmed the crystalline nature of the CuO nanoparticles, with a crystallite size of 14.65 nm for CuO-C and 18.73 nm for CuO-B. The grain sizes of CuO-C (14.09 ± 0.17 nm) and CuO-B (67.88 ± 2.08 nm) were determined using transmission electron microscopy micrographs. Furthermore, the synthesized nanomaterial and the crude ethanolic extract, n-butanol fraction, were examined for their biological potentials namely antioxidant, anti-inflammatory, antimicrobial, and anti-proliferative activity against HeLa cancer cells. Among the synthesized nanomaterials, copper oxide nanoparticles synthesized by utilizing the n-butanol fraction have appeared as a potential biomedical agent. CuO-B has arisen as an antioxidant agent with IC values of 44.63 ± 0.49 µg/mL, 48.49 ± 0.17 µg/mL, and 35.39 ± 0.61 µg/mL for DPPH, FRAP, and reducing power assay, respectively. Furthermore, the significant antibacterial potential of CuO-B against gram-positive (S. aureus MIC 46.88 µg/mL) and gram-negative (K. pneumonia MIC 23.48 µg/mL) bacterial strains cannot be neglected either. Along with this, the IC value (138.07 µg/mL) of CuO-B against HeLa cells proved it to be a potential anticancerous agent. Hence, this novel approach emphasized that these synthesized nanoparticles have tremendous biological potential and can be applied to various fields of agriculture and biomedicine.
由粗乙醇提取物和铁线蕨的正丁醇馏分介导的氧化铜纳米颗粒（CuO）的绿色合成代表了纳米技术中的一种开创性方法，它将生态可持续性与先进功能相结合。这种创新方法利用铁线蕨中存在的天然生物活性化合物来制备CuO纳米颗粒，这些纳米颗粒具有显著的抗氧化、抗炎、抗菌和抗增殖特性。使用多种技术对绿色合成的纳米颗粒进行了表征，XRD证实了CuO纳米颗粒的晶体性质，其中CuO - C的微晶尺寸为14.65纳米，CuO - B的微晶尺寸为18.73纳米。使用透射电子显微镜照片确定了CuO - C（14.09±0.17纳米）和CuO - B（67.88±2.08纳米）的晶粒尺寸。此外，对合成的纳米材料以及粗乙醇提取物、正丁醇馏分进行了生物活性检测，即对HeLa癌细胞的抗氧化、抗炎、抗菌和抗增殖活性检测。在合成的纳米材料中，利用正丁醇馏分合成的氧化铜纳米颗粒已成为一种潜在的生物医学试剂。CuO - B作为抗氧化剂，在DPPH、FRAP和还原力测定中的IC值分别为44.63±0.49微克/毫升、48.49±0.17微克/毫升和35.39±0.61微克/毫升。此外，CuO - B对革兰氏阳性菌（金黄色葡萄球菌MIC为46.88微克/毫升）和革兰氏阴性菌（肺炎克雷伯菌MIC为23.48微克/毫升）的显著抗菌潜力也不容忽视。与此同时，CuO - B对HeLa细胞的IC值（138.07微克/毫升）证明它是一种潜在的抗癌剂。因此，这种新方法强调这些合成的纳米颗粒具有巨大的生物潜力，可应用于农业和生物医学的各个领域。
