Department of Biochemistry and Molecular Biology, Medical School of Ningbo University, Ningbo, China; Zhejiang Provincial Key Laboratory of Pathophysiology, Medical School of Ningbo University, Ningbo, China.
Department of Thoracic Surgery, The Affiliated Ningbo Medical Center Lihuili Eastern Hospital of Medical School of Ningbo University, Ningbo, China.
Clin Chim Acta. 2018 Nov;486:26-33. doi: 10.1016/j.cca.2018.07.026. Epub 2018 Jul 17.
Long noncoding RNAs (lncRNAs) have been proven to be involved in carcinogenesis and to be released into peripheral blood. Our objective was to develop a circulating lncRNA as a novel biomarker to predict lung cancer.
We analyzed the lncRNA expression profile in lung cancer patients by lncRNA array and identified lncRNA XLOC_009167 as a circulating biomarker using qRT-PCR in whole blood of lung cancer patients. The diagnostic value of was analyzed by area under curve (AUC) and the receiver operating characteristic (ROC) test.
LncRNA XLOC_009167 was screened as a candidate biomarker for lung cancer and was up-regulated in both lung cancer tissues and cell lines. Notably, lncRNA XLOC_009167 in whole blood of lung cancer patients was highly expressed as compared with that in healthy controls or in patients with pneumonia. The values of AUC of lung cancer vs. healthy controls, and that of lung cancer vs. pneumonia were 0.7398 (95%CI = 0.6493 to 0.8303) and 0.7005 (95%CI = 0.5771 to 0.8240), respectively. Intriguingly, the ROC showed lncRNA XLOC_009167 was a better diagnostic potential compared to the traditional biomarkers (CYFR21-1, NSE and CA72-4), and the circulating lncRNA XLOC_009167 was found to be stable in whole blood under different conditions.
LncRNA XLOC_009167 could serve as a novel diagnostic biomarker to distinguish lung cancer from benign lung disease and healthy controls.
长链非编码 RNA（lncRNA）已被证明参与了癌症的发生，并被释放到外周血中。我们的目的是开发一种循环 lncRNA 作为一种新的生物标志物来预测肺癌。
我们通过 lncRNA 芯片分析了肺癌患者的 lncRNA 表达谱，并使用 qRT-PCR 法在肺癌患者的全血中鉴定出 lncRNA XLOC_009167 作为一种循环生物标志物。通过曲线下面积（AUC）和受试者工作特征（ROC）检验分析其诊断价值。
lncRNA XLOC_009167 被筛选为肺癌的候选生物标志物，在肺癌组织和细胞系中均上调。值得注意的是，与健康对照组或肺炎患者相比，肺癌患者全血中的 lncRNA XLOC_009167 表达水平较高。肺癌与健康对照组相比，以及肺癌与肺炎相比的 AUC 值分别为 0.7398（95%CI=0.6493 至 0.8303）和 0.7005（95%CI=0.5771 至 0.8240）。有趣的是，ROC 显示 lncRNA XLOC_009167 比传统标志物（CYFR21-1、NSE 和 CA72-4）具有更好的诊断潜力，并且在不同条件下，循环 lncRNA XLOC_009167 在全血中表现出稳定性。
lncRNA XLOC_009167 可作为一种新型诊断生物标志物，用于区分肺癌与良性肺部疾病和健康对照组。
