Elson C J
Eur J Immunol. 1977 Jan;7(1):6-10. doi: 10.1002/eji.1830070103.
These experiments demonstrate that the response of B lymphocytes to particulate antigen in vivo can depend on their stage of differentiation: B lymphocytes differentiating from stem cells were rendered tolerant, while mature B lymphocytes were primed under the same conditions. Lethally irradiated mice of one allotype were repopulated with 13-15 day fetal liver cells from congenic mice bearing another allotype and the effect of antigen on the emergence of responsive B cells determined. B cells, descended from the fetal liver inoculum (identified by their allotype) produced an antibody response to antigen in the presence of additional T cells, 15 days after transfer. Such a response could be prevented by injecting the recipient with alum-precipitated antigen (but not with deaggregated antigen) shortly after irradiation and reconstitution. This unresponsive state was specific and independent of afferent suppressor mechanisms. Mature B cells, on the other hand, were shown to be primed by alum precipitated antigen in irradiated reconstituted hosts. It was concluded that a fundamental difference exists between the responsiveness of mature and differentiating B lymphocytes. Tolerance in differentiating B lymphocytes is discussed in relation to the acquisition of self-tolerance.
这些实验表明，B淋巴细胞在体内对颗粒性抗原的反应可能取决于其分化阶段：从干细胞分化而来的B淋巴细胞表现出耐受，而成熟的B淋巴细胞在相同条件下则被致敏。用来自携带另一种同种异型的同基因小鼠的13 - 15天龄胎肝细胞对一种同种异型的致死性照射小鼠进行再填充，并确定抗原对反应性B细胞出现的影响。移植后15天，源自胎肝接种物（通过其同种异型鉴定）的B细胞在存在额外T细胞的情况下对抗原产生抗体反应。通过在照射和再填充后不久给受体注射明矾沉淀抗原（但不是解聚抗原），可以阻止这种反应。这种无反应状态是特异性的，且与传入抑制机制无关。另一方面，成熟的B细胞在照射后的再填充宿主中被明矾沉淀抗原致敏。得出的结论是，成熟和分化中的B淋巴细胞的反应性存在根本差异。关于分化中的B淋巴细胞的耐受性，结合自身耐受性的获得进行了讨论。
