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即食食品中的微生物存在情况及抗生素耐药性。

Microbial occurrence and antibiotic resistance in ready-to-go food items.

作者信息

Cole Mariah L, Singh Om V

机构信息

1Division of Biological and Health Sciences, University of Pittsburgh, Bradford, PA 16701 USA.

Present Address: Technology Science Group, 1150 18th Street NW, Suite 1000, Washington, DC 20036 USA.

出版信息

J Food Sci Technol. 2018 Jul;55(7):2600-2609. doi: 10.1007/s13197-018-3180-4. Epub 2018 Apr 28.

Abstract

Foodborne pathogens, such as and , are commonly prevalent in contaminated food products seen through annual food recalls. Excessive use of antibiotics through the past few decades has led to a multitude of antibiotic resistant bacteria, including foodborne pathogens. We investigated microbial occurrence and their antibiotics resistances in ready-to-go food items, i.e. canned food, bagged food, and baby food. A total of 112 isolates were isolated from varying food items, and 21 of these isolates were identified through 16S rRNA sequencing revealing sp., sp. and sp. Bagged food items showed the most microbial diversity as well as the largest colony forming unit (log 20-25 CFU/g). Isolates showed antibiotic resistance to ampicillin, streptomycin, chloramphenicol, and kanamycin at concentrations of 100, 500, and 1000 µg/mL. 57% isolates were ampicillin resistance followed by kanamycin (26%). A variety of microorganisms present in ready-to-go food items may not be pathogenic, however their occurrence and multiple antibiotic resistance (MAR) poses risk of transferring their genes to foodborne pathogens.

摘要

食源性病原体,如[具体病原体1]和[具体病原体2],通过年度食品召回可以看出,在受污染的食品中普遍存在。在过去几十年中,抗生素的过度使用导致了大量的耐药细菌,包括食源性病原体。我们调查了即食食品(即罐头食品、袋装食品和婴儿食品)中的微生物存在情况及其抗生素耐药性。总共从不同的食品中分离出112株菌株,其中21株通过16S rRNA测序鉴定,结果显示有[具体菌属1]属、[具体菌属2]属和[具体菌属3]属。袋装食品显示出最多的微生物多样性以及最大的菌落形成单位(对数20 - 25 CFU/g)。分离株对浓度为100、500和1000μg/mL的氨苄青霉素、链霉素、氯霉素和卡那霉素表现出抗生素耐药性。57%的分离株对氨苄青霉素耐药,其次是卡那霉素(26%)。即食食品中存在各种微生物,它们可能不具有致病性,然而它们的存在和多重抗生素耐药性(MAR)带来了将其基因转移到食源性病原体的风险。

相似文献

1
Microbial occurrence and antibiotic resistance in ready-to-go food items.即食食品中的微生物存在情况及抗生素耐药性。
J Food Sci Technol. 2018 Jul;55(7):2600-2609. doi: 10.1007/s13197-018-3180-4. Epub 2018 Apr 28.

本文引用的文献

1
Human microbiome versus food-borne pathogens: friend or foe.人类微生物组与食源性病原体:敌友关系。
Appl Microbiol Biotechnol. 2016 Jun;100(11):4845-63. doi: 10.1007/s00253-016-7523-7. Epub 2016 Apr 22.
9
Finding alternatives to antibiotics.寻找抗生素的替代品。
Ann N Y Acad Sci. 2014 Sep;1323:91-100. doi: 10.1111/nyas.12468. Epub 2014 Jun 20.

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