Key Laboratory of Horticulture Science for Southern Mountainous Regions, Ministry of Education, Southwest University, No. 2 Tiansheng Road, Beibei, Chongqing 400715, China.
Academy of Agricultural Sciences, Southwest University, Chongqing 400715, China.
Int J Mol Sci. 2018 Jul 26;19(8):2181. doi: 10.3390/ijms19082181.
plants exhibit both high biomass productivity and high rates of heavy metal absorption. Metallothionein (MT) proteins are low molecular weight, cysteine-rich, metal-binding proteins that play crucial roles in protecting plants from heavy metal toxicity. However, to date, MT proteins have not been systematically characterized in . In this study, we identified 60 MTs from and five species. All the MT family genes from Brassica are closely related to MTs, encoding putative proteins that share similar functions within the same clades. Genome mapping analysis revealed high levels of synteny throughout the genome due to whole genome duplication and segmental duplication events. We analyzed the expression levels of 16 MTs () by RNA-sequencing and real-time RT-PCR (RT-qPCR) analysis in plants under As stress. These genes exhibited different expression patterns in various tissues. Our results suggest that plays a key role in the response to As stress in . This study provides insight into the phylogeny, origin, and evolution of MT family members in , laying the foundation for further studies of the roles of MT proteins in these important crops.
植物表现出高生物质生产力和高重金属吸收率。金属硫蛋白 (MT) 蛋白是低分子量、富含半胱氨酸、金属结合蛋白，在保护植物免受重金属毒性方面发挥着关键作用。然而，迄今为止，尚未在 中系统地描述 MT 蛋白。在这项研究中，我们从 中鉴定出 60 种 MT，从 中鉴定出 5 种 MT。来自芸薹属的所有 MT 家族基因都与 MT 密切相关，编码具有相似功能的假定蛋白，在同一进化枝内具有相似的功能。基因组图谱分析显示，由于全基因组复制和片段复制事件，整个基因组中的同线性水平很高。我们通过 RNA-seq 和实时 RT-PCR (RT-qPCR) 分析分析了 16 种 MTs () 在 As 胁迫下植物中的表达水平。这些基因在不同组织中表现出不同的表达模式。我们的研究结果表明，在 As 胁迫响应中发挥关键作用。本研究深入了解了 MT 家族成员在 中的系统发育、起源和进化，为进一步研究 MT 蛋白在这些重要作物中的作用奠定了基础。
