In Silico Study of the (/ Homologs) Gene Family and Expression Analysis in Response to PGPR Bacteria and Salinity in .

Among several mechanisms involved in the plant stress response, synthesis of guanosine tetra and pentaphosphates (alarmones), homologous to the bacterial stringent response, is of crucial importance. Plant alarmones affect, among others, photosynthetic activity, metabolite accumulation, and nutrient remobilization, and thus regulate plant growth and development. The plant (/ homolog) genes, that encode synthetases and/or hydrolases of alarmones, have been characterized in a limited number of plant species, e.g., , and . Here, we used dry-to-wet laboratory research approaches to characterize family genes in the polyploid plant . There are 12 genes in the genome of rapeseed that belong to four types of genes: 6 , 2 , 3 , and 1 . genes contain 13-24 introns in , 2-6 introns in , 1-6 introns in , and 2-3 introns in the genes. In the promoter regions of the genes, we showed the presence of regulatory elements of the response to light, plant hormones, plant development, and abiotic and biotic stresses. The wet-lab analysis showed that expression of genes is generally not significantly affected by salt stress, but that the presence of PGPR bacteria, mostly of sp., increased the expression of significantly. The obtained results show that genes are differently affected by biotic and abiotic factors, which indicates their different functions in plants.