Shanghai Center for Plant Stress Biology, CAS Center of Excellence in Molecular Plant Sciences, Chinese Academy of Sciences, Shanghai, China.
University of Chinese Academy of Sciences, Beijing, China.
Plant Biotechnol J. 2019 Feb;17(2):499-504. doi: 10.1111/pbi.12993. Epub 2018 Oct 5.
Base editing is a novel genome editing strategy that enables irreversible base conversion at target loci without the need for double stranded break induction or homology-directed repair. Here, we developed new adenine and cytosine base editors with engineered SpCas9 and SaCas9 variants that substantially expand the targetable sites in the rice genome. These new base editors can edit endogenous genes in the rice genome with various efficiencies. Moreover, we show that adenine and cytosine base editing can be simultaneously executed in rice. The new base editors described here will be useful in rice functional genomics research and will advance precision molecular breeding in crops.
碱基编辑是一种新型的基因组编辑技术，可在无需双链断裂诱导或同源定向修复的情况下，实现靶向位点的不可逆碱基转换。在此，我们开发了新型的腺嘌呤碱基编辑器和胞嘧啶碱基编辑器，其所用的 SpCas9 和 SaCas9 变体经过了工程改造，从而极大地扩展了水稻基因组中的靶向位点。这些新的碱基编辑器可以以不同的效率编辑水稻基因组中的内源性基因。此外，我们还表明，腺嘌呤碱基编辑和胞嘧啶碱基编辑可以在水稻中同时进行。本文所描述的新型碱基编辑器将有助于水稻功能基因组学研究，并将推动作物的精准分子育种。
