Murata K, Fisch U
Ann Otol Rhinol Laryngol. 1977 Mar-Apr;86(2 pt. 1):164-71. doi: 10.1177/000348947708600205.
A microelectrode with a tip of 100micron permitting recording of the oxygen tension in the perilymphatic space according to the polarographic principle and having a minimal drift of 1-25% per hour has been developed. The effects of apnea, hypo- and hyperventilation as well as those of inhalation of pure oxygen, and CO2 upon the perilymphatic PO2 have been measured by placing the electrode in the perilymph through the fenestrated stapedial footplate of 87 adult cats. The correlation between the arterial PO2 and the perilymphatic PO2 is so close that even hypo- or hyperventilation in presence of air does influence the oxygen content of the perilymphatic space. In view of the effect of the smallest accumulation of alveolar CO2, particular attention has to be paid to the system used for respiration of the experimental animal, when determining the action of drugs or inhaled gas mixtures on the oxygenation of the inner ear fluids. The measurement of the perilymphatic oxygen tension also indicates that the rate of blood flow cannot be used to deduct with accuracy the actual degree of oxygenation of the inner ear fluids.
已研制出一种微电极，其尖端为100微米，可根据极谱原理记录外淋巴间隙中的氧张力，每小时的最小漂移率为1 - 25%。通过将该电极经87只成年猫的镫骨足板开窗置于外淋巴中，测量了呼吸暂停、通气不足和通气过度以及吸入纯氧和二氧化碳对外淋巴PO2的影响。动脉PO2与外淋巴PO2之间的相关性非常密切，以至于即使在有空气存在的情况下通气不足或过度通气也会影响外淋巴间隙的氧含量。鉴于肺泡二氧化碳的最小积聚效应，在确定药物或吸入气体混合物对内耳液体氧合作用时，必须特别注意用于实验动物呼吸的系统。外淋巴氧张力测量还表明，不能用血流速率准确推断内耳液体的实际氧合程度。
