Ajayi Abayomi M, Ben-Azu Benneth, Onasanwo Samuel A, Adeoluwa Olusegun, Eduviere Anthony, Ademowo Olusegun G
Department of Pharmacology & Therapeutics, College of Medicine, University of Ibadan, Ibadan, Nigeria.
Department of Physiology, College of Medicine, University of Ibadan, Ibadan, Nigeria.
Drug Res (Stuttg). 2019 Feb;69(3):151-158. doi: 10.1055/a-0654-5042. Epub 2018 Jul 31.
L. leaves has been traditionally used for management of febrile illnesses and symptoms typified of sickness behavior. In this work we investigated the modulatory effect of flavonoid-rich fraction of leaves (EAFO) on sickness behavior, inflammatory and oxidative stress responses in LPS-challenged mice.
leaf was first extracted with n-hexane, chloroform and methanol, and EAFO was obtained by ethylacetate partitioning of a sequentially resultant methanol extract. The effect of EAFO (25-100 mg/kg) on acute LPS-induced neurobehavioral impairment in an open field test (OFT) and depressive-like behavior in forced swimming test (FST) was investigated. Serum nitrite and TNF-α, as well as myeloperoxidase (MPO), malondialdehyde (MDA), and reduced glutathione (GSH) levels were determined in liver and brain tissues.
EAFO prevented the reduction in locomotor and rearing activity in OFT and the increase in immobility time in FST. The fraction significantly attenuated the elevation of serum TNF- α and nitrite levels. EAFOg reversed LPS-induced increase in MDA, MPO, and nitrite levels and attenuated GSH depletion in liver and brain tissues of mice.
Flavonoid-rich fraction of leaf demonstrated significant modulation of LPS-induced sickness behavior, inflammatory and oxidative stress response in mice. This suggests an important therapeutic strategy in slowing down LPS-mediated hepatic and neuronal disease processes.
传统上，[植物名称]叶被用于治疗发热性疾病和类似疾病行为的症状。在本研究中，我们研究了[植物名称]叶富含黄酮类成分（EAFO）对脂多糖（LPS）刺激小鼠的疾病行为、炎症和氧化应激反应的调节作用。
首先用正己烷、氯仿和甲醇对[植物名称]叶进行提取，然后通过乙酸乙酯对依次得到的甲醇提取物进行分配得到EAFO。研究了EAFO（25 - 100mg/kg）在旷场试验（OFT）中对急性LPS诱导的神经行为损伤以及在强迫游泳试验（FST）中对抑郁样行为的影响。测定了肝脏和脑组织中的血清亚硝酸盐和肿瘤坏死因子-α（TNF-α）水平，以及髓过氧化物酶（MPO）、丙二醛（MDA）和还原型谷胱甘肽（GSH）水平。
EAFO可防止OFT中运动和站立活动的减少以及FST中不动时间的增加。该成分显著减轻了血清TNF-α和亚硝酸盐水平的升高。EAFO可逆转LPS诱导的小鼠肝脏和脑组织中MDA、MPO和亚硝酸盐水平的升高，并减轻GSH的消耗。
[植物名称]叶富含黄酮类成分对LPS诱导的小鼠疾病行为、炎症和氧化应激反应具有显著调节作用。这提示了一种减缓LPS介导的肝脏和神经疾病进程的重要治疗策略。
