Omidbakhsh Ameneh, Saeedi Mohsen, Khoshnia Masoud, Marjani Abdoljalal, Hakimi Safoura
Student Research Committee, Gorgan Faculty of Medicine, Golestan University of Medical Sciences, Gorgan, Golestan province, Iran.
Stem Cell Research Center, Gorgan Faculty of Medicine, Golestan University of Medical Sciences, Gorgan, Golestan province, Iran.
Open Biochem J. 2018 Jun 29;12:78-86. doi: 10.2174/1874091X01812010078. eCollection 2018.
MicroRNAs (miRNAs) can regulate various genes after binding to target mRNAs. Studies on Inflammatory Bowel Disease (IBD) in relation with miRNA are much less shown. The aim of the present study was to assess the expression patterns of microRNA 106a and microRNA 362-3p in peripheral blood samples of Inflammatory Bowel Disease (IBD) patients including Crohn's Disease(CD) and Ulcerative Colitis (UC).
This study consisted of 32 CD, 32 UC patients and 32 controls. The expression level of the micro-RNAs -106a and -362-3p was determined using reverse transcription and real-time RT-PCR.
Our findings showed that MiR-106a and miR-362-3p are expressed at significantly higher levels in the peripheral blood from patients with CD and UC compared to controls. MiR-106a and miR-362-3p expression are also different in the peripheral blood of patients regarding the activity score of the disease. There were significant differences of miR362-3p in active UC relative to inactive UC.
Altogether our findings suggest that miR-106a and miR-363-3p can play an important role in the pathogenesis of IBD. The differences in expression of miR106a and miR362-3p in peripheral blood of the UC and CD patients in an active phase in comparison to inactive disease suggest that these miRNAs may be useful as potential biomarkers for diagnosis and monitoring the disease activity.
微小RNA（miRNA）与靶mRNA结合后可调控多种基因。关于miRNA与炎症性肠病（IBD）的研究较少。本研究旨在评估微小RNA 106a和微小RNA 362 - 3p在炎症性肠病（IBD）患者外周血样本中的表达模式，这些患者包括克罗恩病（CD）和溃疡性结肠炎（UC）。
本研究纳入32例CD患者、32例UC患者和32例对照。使用逆转录和实时RT - PCR测定微小RNA - 106a和 - 362 - 3p的表达水平。
我们的研究结果表明，与对照组相比，CD和UC患者外周血中MiR - 106a和miR - 362 - 3p的表达水平显著更高。MiR - 106a和miR - 362 - 3p在患者外周血中的表达也因疾病活动评分而异。活动期UC患者中miR362 - 3p相对于非活动期UC患者有显著差异。
我们的研究结果表明，miR - 106a和miR - 363 - 3p在IBD发病机制中可能起重要作用。与疾病非活动期相比，活动期UC和CD患者外周血中miR106a和miR362 - 3p表达的差异表明，这些miRNA可能作为诊断和监测疾病活动的潜在生物标志物。
