Wang Nan-Nan, Sang Jin, Wang Xiao-Qun, Li Ying-Xiu, Du Zong-Jun
1College of Marine Science, Shandong University, Weihai, 264209, PR China.
2State Key Laboratory of Microbial Technology, Shandong University, Jinan 250100, PR China.
Int J Syst Evol Microbiol. 2018 Oct;68(9):3169-3174. doi: 10.1099/ijsem.0.002959. Epub 2018 Aug 9.
A novel Gram-stain-negative, whitish-yellow, rod-shaped, non-pigmented, aerobic, oxidase-positive and catalase-positive bacterium, designated PX7, was isolated from coastal sediment of Xiaoshi Island, Weihai, China (37° 31' 36″ N, 122° 00' 58″ E). Strain PX7 grew optimally at 30 °C, at pH 7.0-7.5 and in the presence of 3.0 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain PX7 formed a robust clade with members of the genus Primorskyibacter and was closely related to Primorskyibacter sedentarius, Primorskyibacter aestuariivivens and Primorskyibacter insulae with 96.5, 96.2 and 95.1 % sequence similarities, respectively. The sole respiratory quinone of strain PX7 was ubiquinone-10, and the dominant fatty acid was C18 : 1ω7c (80.2 %). The major polar lipids were phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine, one unidentified aminolipid, one unidentified phospholipid and one unidentified lipid. The DNA G+C content of strain PX7 was 60.2 mol%. Based on the combination of phylogenetic analyses, phenotypic and chemotaxonomic data, strain PX7 is considered to represent a novel species within the genus Primorskyibacter in the family Rhodobacteraceae, for which the name Primorskyibacter marinus sp. nov. is proposed. The type strain of the new species is PX7 (=KCTC 42952=MCCC 1H00196).
从中国威海小石岛（北纬37°31′36″，东经122°00′58″）的海岸沉积物中分离出一株新型革兰氏阴性、微黄白色、杆状、无色素、需氧、氧化酶阳性且过氧化氢酶阳性的细菌，命名为PX7。菌株PX7在30 °C、pH 7.0 - 7.5以及3.0%（w/v）NaCl存在的条件下生长最佳。基于16S rRNA基因序列的系统发育分析表明，菌株PX7与Primorskyibacter属的成员形成了一个稳健的分支，并且与Primorskyibacter sedentarius、Primorskyibacter aestuariivivens和Primorskyibacter insulae密切相关，序列相似性分别为96.5%、96.2%和95.1%。菌株PX7的唯一呼吸醌为泛醌-10，主要脂肪酸为C18 : 1ω7c（80.2%）。主要极性脂为磷脂酰甘油、磷脂酰胆碱、磷脂酰乙醇胺、一种未鉴定的氨基脂、一种未鉴定的磷脂和一种未鉴定的脂类。菌株PX7的DNA G+C含量为60.2 mol%。基于系统发育分析、表型和化学分类数据的综合结果，菌株PX7被认为代表红杆菌科Primorskyibacter属内的一个新物种，为此提出名为Primorskyibacter marinus sp. nov.的新名称。该新物种的模式菌株为PX7（=KCTC 42952 = MCCC 1H00196）。
