Marine College, Shandong University, Weihai, 264209, Shandong, People's Republic of China.
Department of Blood Transfusion, Weihai Municipal Hospital, Cheeloo College of Medicine, Shandong University, Weihai, 264200, Shandong, People's Republic of China.
Arch Microbiol. 2021 Sep;203(7):4493-4498. doi: 10.1007/s00203-021-02434-z. Epub 2021 Jun 19.
A Gram-stain-negative, oval or short rod-shaped, non-motile, aerobic bacterium, designated strain S1109L, was isolated from a marine sediment in Weihai, PR China. Cells were oxidase positive and catalase positive. Growth of strain S1109L occurred at 10-40 °C (optimum, 30-33 °C), pH 6.5-10.0 (optimum, 7.0-8.0) and in the presence of 1-21% (optimum, 4-6%) (w/v) NaCl. 16S rRNA gene sequence phylogeny indicated that strain S1109L was associated with the genus Pontibaca of the family Rhodobacteraceae because it showed the highest sequence similarity to Pontibaca methylaminivorans KCTC 22497 (97.5%). The average nucleotide identity (ANI) and the digital DNA-DNA hybridization (dDDH) scores between strain S1109L and Pontibaca methylaminivorans KCTC 22497 were 74.6% and 18.7%. The major cellular fatty acids of strain S1109L were C cyclo ω8c and C ω7c. The polar lipids profiles of strain S1109L were phosphatidylglycerol, phosphatidylcholine, phosphatidylethanolamine and two unidentified lipids. Strain S1109L contained ubiquinone-10 as the major respiratory quinone. The genomic DNA G + C content was 55.9 mol%. On the basis of the evidence presented in this study, strain S1109L is considered to represent a novel species of the genus Pontibaca, for which the name Pontibaca salina sp. nov. is proposed. The type strain of is S1109L (= KCTC 82411 = MCCC 1H00441).
一株革兰氏阴性、短杆状或椭圆形、不运动、需氧细菌，被命名为 S1109L 菌株，从中国威海的海洋沉积物中分离得到。细胞氧化酶阳性，过氧化氢酶阳性。S1109L 菌株的生长温度为 10-40°C（最佳温度为 30-33°C），pH 值为 6.5-10.0（最佳 pH 值为 7.0-8.0），并且可以在 1-21%（最佳为 4-6%）（w/v）的 NaCl 浓度下生长。16S rRNA 基因序列系统发育分析表明，S1109L 菌株与红杆菌科 Rhodobacteraceae 属的 Pontibaca 属有关，因为它与 Pontibaca methylaminivorans KCTC 22497（97.5%）的序列相似性最高。S1109L 菌株与 Pontibaca methylaminivorans KCTC 22497 的平均核苷酸同一性（ANI）和数字 DNA-DNA 杂交（dDDH）分数分别为 74.6%和 18.7%。S1109L 菌株的主要细胞脂肪酸为 C cyclo ω8c 和 C ω7c。S1109L 菌株的极性脂图谱由磷脂酰甘油、磷脂酰胆碱、磷脂酰乙醇胺和两种未鉴定的脂组成。S1109L 菌株含有泛醌-10 作为主要呼吸醌。基因组 DNA G+C 含量为 55.9mol%。基于本研究提供的证据，S1109L 菌株被认为代表了 Pontibaca 属的一个新种，因此建议将其命名为 Pontibaca salina sp. nov.。其模式菌株为 S1109L（=KCTC 82411=MCCC 1H00441）。
