Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, 300072, PR China.
Tianjin Key Laboratory for Modern Drug Delivery & High-Efficiency, School of Pharmaceutical Science and Technology, Tianjin University, Tianjin, 300072, PR China.
Carbohydr Polym. 2018 Oct 15;198:581-588. doi: 10.1016/j.carbpol.2018.06.120. Epub 2018 Jun 30.
Polysaccharides and flavonoids co-existed in corn silk (Maydis stigma) could interact inevitably during processing and digestion. In this study, the binding interaction between three polysaccharides with different molecular weight and flavonoids from corn silk was characterized using molecular dynamic and thermodynamic simulation. And the corn silk polysaccharides-flavonoids complex (CSP - CSF complex) was characterized using fourier transform infrared (FT-IR) spectra, circular dichroism (CD), scanning electron microscope (SEM) and differential scanning calorimetry (DSC). The three polysaccharides from corn silk showed the molecular weight distributions of 43.3 kDa, 61.3 kDa and 106.6 kDa, respectively, and they had the same monosaccharide types with different ratios. The adsorption of flavonoids to polysaccharides might be mostly driven by van der Waals forces and hydrogen bonding, and it could be described through various isothermal models and thermodynamic equations such as Langmuir, Freundlich equations and Clausius-Clapeyron equation. This type of interactions could improve the biological activities of polysaccharides such as α-amylase and α-glucosidase inhibition.
玉米须（Maydis stigma）中的多糖和类黄酮在加工和消化过程中不可避免地会相互作用。在这项研究中，使用分子动力学和热力学模拟研究了三种不同分子量的多糖与玉米须中类黄酮之间的结合相互作用。使用傅里叶变换红外（FT-IR）光谱、圆二色性（CD）、扫描电子显微镜（SEM）和差示扫描量热法（DSC）对玉米须多糖-类黄酮复合物（CSP-CSF 复合物）进行了表征。玉米须中的三种多糖的分子量分布分别为 43.3kDa、61.3kDa 和 106.6kDa，它们具有相同的单糖类型，但比例不同。类黄酮对多糖的吸附可能主要是由范德华力和氢键驱动的，可以通过各种等温模型和热力学方程来描述，如朗缪尔、弗伦德利希方程和克劳修斯-克拉佩龙方程。这种相互作用可以提高多糖的生物活性，如对α-淀粉酶和α-葡萄糖苷酶的抑制作用。
