Peng Dan, Tan Xiaofeng, Zhang Lin, Yuan Deyi, Lin Jianzhong, Liu Xuanming, Jiang Yueqiao, Zhou Bo
Faculty of Life Science and Technology, Central South University of Forestry and Technology, 410004, Changsha, China.
Forestry Biotechnology Hunan Key Laboratories, 410004, Changsha, China.
Breed Sci. 2018 Jun;68(3):326-335. doi: 10.1270/jsbbs.17126. Epub 2018 Jul 5.
Branching is a predominant element in the plant architecture of L. and represents an important determinant of seed yield. (), a novel DHHC-type zinc finger protein gene, was reported to regulate rice plant architecture by altering the tillering. However, whether heterologous overexpression of the gene from the monocot rice into the dicot L. would have the same effect on branching or seed yield is unknown. In this study, the DHHC-type zinc finger protein gene was determined to have sulfur acyl transferase activity in the yeast mutant in a complementation experiment. Heterologously expressing transgenic L. plants were obtained using the -mediated floral-dip transformation method. As anticipated, transgenic plants exhibited branching and seed yield. Compared with non-transgenic plants, transgenic plants had increased primary branches (1.58-1.76-fold) and siliques (1.86-1.89-fold), resulting in a significant increase in seed yield (around 2.39-2.51-fold). Therefore, overexpression of the sulfur acyl transferase gene in L. could be used to increase seed yield and produce excellent varieties.
分枝是番茄植株结构中的一个主要元素，也是种子产量的一个重要决定因素。据报道，一个新的DHHC型锌指蛋白基因（）通过改变分蘖来调控水稻植株结构。然而，将单子叶水稻中的该基因异源过表达导入双子叶番茄中是否会对分枝或种子产量产生相同的影响尚不清楚。在本研究中，通过互补实验确定DHHC型锌指蛋白基因在酵母突变体中具有硫酰基转移酶活性。使用介导的花浸染转化方法获得了异源表达该基因的转基因番茄植株。正如预期的那样，转基因植株表现出分枝和种子产量增加。与非转基因植株相比，转基因植株的主枝（增加了1.58 - 1.76倍）和角果（增加了1.86 - 1.89倍）增多，导致种子产量显著增加（约2.39 - 2.51倍）。因此，在番茄中过表达硫酰基转移酶基因可用于提高种子产量并培育优良品种。
