Monobe Marina M, Junior João P Araujo, Lunsford Kari V, Silva Rodrigo C, Bulla Camilo
Department of Veterinary Clinics, School of Veterinary Medicine and Animal Science.
Department of Microbiology and Immunology, Biosciences Institute, Sao Paulo State University (UNESP), Botucatu, Brazil.
Vet Med (Auckl). 2015 Apr 13;6:111-117. doi: 10.2147/VMRR.S72373. eCollection 2015.
To date, a 4-bp deletion in the MDR1 gene has been detected in more than ten dog breeds, as well as in mixed breed dogs, in several countries, however information regarding this mutation in dogs from Brazil is lacking. For this reason, 103 Collies, 77 Border Collies, 76 Shetland Sheepdogs, 20 Old English Sheepdogs, 55 German Shepherds, 16 Australian Shepherds, and 53 Whippets from Brazil were screened for the presence of the mutation. The heterozygous mutated genotype, MDR1 (+/-), frequency found for Collies, Australian Shepherd, and Shetland Sheepdog was 50.5% (95% CI =41.1%-59.9%), 31.3% (95% CI =8.6%-53.2%), and 15.8% (95% CI =7.7%-23.9%), respectively. Homozygous mutated genotype, MDR1 (-/-), was detected only in Collies 35.9%. The MDR1 allele mutant frequency found for Collies, Australian Shepherd, and Shetland Sheepdog was 61.2% (95% CI =54.8%-67.5%), 15.6% (95% CI =3.1%-28.2%), and 7.9% (95% CI =3.7%-12.1%), respectively. Additionally, even free of the mutant allele, the maximum mutant prevalence (MMP) in that population, with 95% CI, was 3.8%, 5.2%, 5.4%, and 13.8% for Border Collies, German Shepherds, Whippets, and Old English Sheepdogs, respectively. In this way, this information is important, not only for MDR1 genotype-based breeding programs and international exchange of breeding animals of predisposed breeds, but also for modification of drug therapy for breeds at risk.
迄今为止，在多个国家的十多个犬种以及混血犬中都检测到了MDR1基因中的一个4碱基对缺失，然而巴西犬类中关于这种突变的信息却很缺乏。因此，对来自巴西的103只柯利牧羊犬、77只边境牧羊犬、76只设得兰牧羊犬、20只古代英国牧羊犬、55只德国牧羊犬、16只澳大利亚牧羊犬和53只惠比特犬进行了该突变的筛查。柯利牧羊犬、澳大利亚牧羊犬和设得兰牧羊犬的杂合突变基因型MDR1（+/-）频率分别为50.5%（95%置信区间=41.1%-59.9%）、31.3%（95%置信区间=8.6%-53.2%）和15.8%（95%置信区间=7.7%-23.9%）。仅在35.9%的柯利牧羊犬中检测到纯合突变基因型MDR1（-/-）。柯利牧羊犬、澳大利亚牧羊犬和设得兰牧羊犬的MDR1等位基因突变频率分别为61.2%（95%置信区间=54.8%-67.5%）、15.6%（95%置信区间=3.1%-28.2%）和7.9%（95%置信区间=3.7%-12.1%）。此外，即使没有突变等位基因，边境牧羊犬、德国牧羊犬、惠比特犬和古代英国牧羊犬群体中的最大突变患病率（MMP）及95%置信区间分别为3.8%、5.2%、5.4%和13.8%。因此，这些信息不仅对于基于MDR1基因型的育种计划和易患品种种畜的国际交换很重要，而且对于有风险品种的药物治疗调整也很重要。
