Malfoy B, Rousseau N, Vogt N, Viegas-Pequignot E, Dutrillaux B, Leng M
Nucleic Acids Res. 1986 Apr 25;14(8):3197-214. doi: 10.1093/nar/14.8.3197.
The purpose of this work was to analyse at the molecular level the DNA recognized by the antibodies to Z-DNA in in situ experiments. Antibodies to Z-DNA interact strongly with R-band positive heterochromatic segments of fixed metaphase chromosomes of Cebus (Viegas-Pequignot et al., 1983). These segments are constituted of a satellite DNA the repeat unit of which is about 1520 base pairs long. The base sequence of the repeat unit has been determined. It contains a (AC)n rich region which, in vitro, adopts the Z conformation under topological constraints. Experiments with nuclei suggest that this sequence is not predominantly in the Z conformation in vivo. The polymorphic structure of the (AC)n rich region argues for an active recombination sequence.
这项工作的目的是在分子水平上分析原位实验中被Z-DNA抗体识别的DNA。Z-DNA抗体与僧帽猴固定中期染色体的R带阳性异染色质区段强烈相互作用（维加斯-佩基诺特等人，1983年）。这些区段由一种卫星DNA构成，其重复单元长度约为1520个碱基对。已确定了重复单元的碱基序列。它包含一个富含(AC)n的区域，在体外，该区域在拓扑限制下会采用Z构象。细胞核实验表明，该序列在体内并非主要处于Z构象。富含(AC)n区域的多态结构表明其为一个活跃的重组序列。
Zotero 插件
