Ishikawa F, Takaku F, Hayashi K, Nagao M, Sugimura T
Proc Natl Acad Sci U S A. 1986 May;83(10):3209-12. doi: 10.1073/pnas.83.10.3209.
Rat c-raf was found to be activated in a transformant obtained with DNA of a hepatocellular carcinoma induced by 2-amino-3-methylimidazo[4,5-f]quinoline. This activated c-raf was cloned in a cosmid vector and actively transforming clones were obtained. Comparison of the restriction maps of this activated c-raf and cloned normal rat c-raf revealed a recombination in the 5'-terminal region of the activated form of this gene. The recombined DNA was shown to be actively transcribed and possibly to form a fused mRNA with c-raf, which is slightly smaller than normal c-raf mRNA. Since this recombination was not detected in the original tumor by Southern blot analysis, it presumably occurred during transfection.
在由2-氨基-3-甲基咪唑[4,5-f]喹啉诱导的肝细胞癌DNA获得的转化体中发现大鼠c-raf被激活。这种激活的c-raf被克隆到粘粒载体中，并获得了具有活性转化能力的克隆。对这种激活的c-raf和克隆的正常大鼠c-raf的限制性图谱进行比较，发现在该基因激活形式的5'-末端区域发生了重组。重组后的DNA被证明能被积极转录，并可能与c-raf形成一种融合mRNA，其大小略小于正常的c-raf mRNA。由于通过Southern印迹分析在原始肿瘤中未检测到这种重组，推测它发生在转染过程中。
