长链非编码 RNA GHET1 的下调抑制了神经胶质瘤细胞的活性。

lncRNA GHET1 down-regulation suppresses the cell activities of glioma.

机构信息

Department of Neurosurgery, The Third Affiliated Hospital of Kunming Medical University, Kunming 650118, Yunnan, China.

Department of Neurosurgery, Yunnan Cancer Hospital, Kunming 650118, Yunnan, China.

出版信息

Cancer Biomark. 2018;23(1):9-22. doi: 10.3233/CBM-171002.

DOI:10.3233/CBM-171002

PMID:30103301

Abstract

Long noncoding RNAs (lncRNAs) are emerging as new modulators in cancer origination and progression. The previous study found lncRNA gastric carcinoma highly expressed transcript 1 (GHET1) was significantly up-regulation in the gastric cancer. In our present study, we studied the GHET1 expression in glioma and its effects to biological activities of glioma in vitro and vivo studies. In the clinical analysis, the results were shown that lncRNA GHET1 was significantly up-regulation and the Numb protein expression was significantly down-regulation in glioma tissues. In vitro and vivo studies, the results were shown that lncRNA GHET1 knock-down had effects to inhibit biological activities by regulation Numb and down-stream genes P53 and MMP2/9. The results suggested that GHET1 is a novel tumor oncogene by Numb in glioma. LncRNA GHET1 may serve as a potential biomarker and therapeutic target for glioma.

摘要

长链非编码 RNA（lncRNA）在癌症的发生和发展中作为新的调节剂而出现。先前的研究发现，胃腺癌高表达转录本 1（GHET1）在胃癌中显著上调。在我们目前的研究中，我们研究了 GHET1 在神经胶质瘤中的表达及其对神经胶质瘤体外和体内生物学活性的影响。在临床分析中，结果表明，lncRNA GHET1 在神经胶质瘤组织中显著上调，Numb 蛋白表达显著下调。在体外和体内研究中，结果表明，lncRNA GHET1 敲低通过调节 Numb 及其下游基因 P53 和 MMP2/9 对抑制生物学活性有影响。结果表明，GHET1 通过 Numb 在神经胶质瘤中是一种新型肿瘤癌基因。lncRNA GHET1 可能作为神经胶质瘤的潜在生物标志物和治疗靶点。