Department of Microbiology and Immunology, Faculty of Pharmacy, Mansoura University, Mansoura 35516, Egypt.
Department of Pharmaceutical Sciences, College of Pharmacy, Princess Nourah bint Abdulrahman University, Riyadh 11671, Saudi Arabia.
IET Nanobiotechnol. 2018 Sep;12(6):741-747. doi: 10.1049/iet-nbt.2017.0213.
Nanoparticles (NPs) are gaining special interest due to their recent applications as antimicrobial agents to defeat the massive threat of resistant pathogens. This study focused on the utilisation of isolate S12 purified from waste discharge soil in the biological synthesis of silver (Ag), selenium (Se), and zinc oxide (ZnO) NPs. The isolate S12 was related to according to 16S rRNA sequence analysis, morphological characteristics, and biochemical reactions. The cell-free supernatant has been used for the synthesis of Ag, Se, and ZnO NPs. The synthesised NPs were characterised using ultraviolet-visible spectroscopy, dynamic light scattering (DLS), transmission electron microscopy, and Fourier transform infrared spectroscopy. The biogenic NPs were evaluated for antimicrobial effects against different Gram-positive and Gram-negative resistant isolates using the broth microdilution method. They showed antibacterial effect against standard and resistant isolates; , ATCC 29213, S1.1, methicillin resistant (MRSA 303, 402 and 807), ATCC 12435, E7, ATCC 51503, K5, K112, PAO1, and P8. This study showed the green synthesis of various NPs using isolate S12 which demonstrated diverse activities against multi-drug resistant isolates.
纳米粒子（NPs）因其最近作为抗菌剂的应用而受到特别关注，以应对耐药病原体的巨大威胁。本研究专注于利用从废水排放土壤中分离出的 S12 纯化物来生物合成银（Ag）、硒（Se）和氧化锌（ZnO） NPs。根据 16S rRNA 序列分析、形态特征和生化反应，S12 分离物与 有关。无细胞上清液已用于合成 Ag、Se 和 ZnO NPs。使用紫外-可见光谱、动态光散射（DLS）、透射电子显微镜和傅里叶变换红外光谱对合成的 NPs 进行了表征。采用肉汤微量稀释法评估生物合成的 NPs 对不同革兰氏阳性和革兰氏阴性耐药分离株的抗菌作用。它们对标准和耐药分离株表现出抗菌作用，包括 、ATCC 29213、S1.1、耐甲氧西林 （MRSA 303、402 和 807）、ATCC 12435、E7、ATCC 51503、K5、K112、PAO1 和 P8。本研究显示了使用 S12 分离物的各种 NPs 的绿色合成，该分离物对多种耐药分离株表现出多种活性。
