稳定期慢性阻塞性肺疾病中与症状相关的痰液微生物群
Symptom-related sputum microbiota in stable chronic obstructive pulmonary disease.
作者信息
Diao Wenqi, Shen Ning, Du Yipeng, Erb-Downward John R, Sun Xiaoyan, Guo Chenxia, Ke Qian, Huffnagle Gary B, Gyetko Margaret R, He Bei
机构信息
Department of Respiratory Medicine, Peking University Third Hospital, Beijing 100191, China,
Division of Pulmonary and Critical Care Medicine, Department of Internal Medicine, University of Michigan Medical School, Ann Arbor, MI, USA.
出版信息
Int J Chron Obstruct Pulmon Dis. 2018 Jul 30;13:2289-2299. doi: 10.2147/COPD.S167618. eCollection 2018.
BACKGROUND
The role of airway microbiota in COPD is highly debated. Symptomology assessment is vital for the management of clinically stable COPD patients; however, the link between symp toms and the airway microbiome is currently unknown.
PURPOSE
The present study aimed to evaluate the relationship among stable COPD patients.
PATIENTS AND METHODS
We conducted pyrosequencing of bacterial 16S rRNA using induced sputum samples in a Han Chinese cohort that included 40 clinically stable COPD patients and 19 healthy controls.
RESULTS
Alterations in community composition and core bacte rial taxa (Neisseria subflava, etc.) were observed in patients with severe symptoms compared with controls. The co-occurrence network indicated that the key microbiota enriched in COPD patients showed higher expression in patients with severe symptoms. The association pattern of symptoms with the sputum microbiome was obviously different from that of lung function in COPD patients.
CONCLUSION
These findings broaden our insights into the relationship between the sputum microbiota and the symptom severity in COPD patients, emphasizing the role of symptoms in the airway microbiome, independent of lung function.
背景
气道微生物群在慢性阻塞性肺疾病（COPD）中的作用备受争议。症状评估对于临床稳定的COPD患者的管理至关重要；然而，目前尚不清楚症状与气道微生物群之间的联系。
目的
本研究旨在评估稳定期COPD患者之间的关系。
患者与方法
我们在一个包含40例临床稳定的COPD患者和19例健康对照的汉族队列中，对诱导痰样本进行细菌16S rRNA焦磷酸测序。
结果
与对照组相比，症状严重的患者群落组成和核心细菌类群（微黄奈瑟菌等）发生了改变。共现网络表明，COPD患者中富集的关键微生物群在症状严重的患者中表达更高。COPD患者症状与痰微生物群的关联模式明显不同于肺功能的关联模式。
结论
这些发现拓宽了我们对COPD患者痰微生物群与症状严重程度之间关系的认识，强调了症状在气道微生物群中的作用，独立于肺功能。
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